18S rRNA is a reliable normalisation gene for real time PCR based on influenza virus infected cells

18S rRNA is a reliable normalisation gene for real time PCR based on influenza virus infected cells

Background: One requisite of quantitative reverse transcription PCR (qRT-PCR) is to normalise the data with an internal reference gene that is invariant regardless of treatment, such as virus infection. Several studies have found variability in the expression of commonly used housekeeping genes, suc...

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Bibliographic Details
Main Authors: Suresh V. Kuchipudi, Meenu Tellabati, Rahul K. Nelli, Gavin A. White, Belinda Baquero Perez, Sujith Sebastian, Marek J. Slomka, Sharon M. Brookes, Ian H. Brown, Stephen P. Dunham, Kin-Chow Chang
Format: Default Article
Published: 2012
Subjects:
Microbiology not elsewhere classified
Mechanical engineering not elsewhere classified
Reference gene
Housekeeping gene
qRT-PCR
Data normalisation
Influenza A viruses
H5N1
H1N1
H2N3
Microbiology
Mechanical Engineering not elsewhere classified
Online Access:https://hdl.handle.net/2134/26719
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https://hdl.handle.net/2134/26719

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