Multi-parameter flow cytometry and cell sorting reveal extensive physiological heterogeneity in Bacillus cereus batch cultures.

Multi-parameter flow cytometry and cell sorting reveal extensive physiological heterogeneity in Bacillus cereus batch cultures.

Based on two staining protocols, DiOC6(3)/ propidium iodide (PI) and RedoxSensor Green (an indicator of bacterial reductase activity)/PI, multiparameter flow cytometry and cell sorting has identified at least four distinguishable physiological states during batch cultures of Bacillus cereus. Further...

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Bibliographic Details
Main Authors: Andrew Want, Helen Hancocks, Stuart M. Stocks, Gerhard Nebe-von-Caron, Christopher Hewitt
Format: Default Article
Published: 2011
Subjects:
Chemical engineering not elsewhere classified
Bacillus cereus
Cell sorting
Bis-(1,3-dibutylbarbituric acid) trimethine oxonol
3,30-Dihexylocarbocyanine iodide
Flow cytometry
Membrane potential
Membrane integrity
Chemical Engineering not elsewhere classified
Online Access:https://hdl.handle.net/2134/8617
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https://hdl.handle.net/2134/8617

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