Induction studies with Escherichia coli expressing recombinant interleukin-13 using multi-parameter flow cytometry

The expression of interleukin -13 following induction with IPTG in E. coli results in metabolic changes as indicated by multi-parameter flow cytometry and traditional methods of fermentation profiling (O2 uptake rate, CO2 evolution rate and optical density measurements). Induction early in the rapid...

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Bibliographic Details
Main Authors: Jennifer O. Shitu, John M. Woodley, Richard Wnek, Michel J. Chartrain, Christopher Hewitt
Format: Default Article
Published: 2009
Subjects:
Chemical engineering not elsewhere classified
Cytoplasmic membrane potential
Escherichia coli
Flow cytometry
Interleukin 13
Recombinant protein
Chemical Engineering not elsewhere classified
Online Access:https://hdl.handle.net/2134/5761
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https://hdl.handle.net/2134/5761

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