Tobacco nuclear DNA contains long tracts of homology to chloroplast DNA

Long tracts of DNA with high sequence homology to chloroplast DNA were isolated from nuclear genomic libraries of Nicotiana tabacum. One lambda EMBL4 clone was characterised in detail and assigned to nuclear DNA. The majority of the 15.5-kb sequence is greater than 99% homologous with its chloroplas...

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Bibliographic Details
Published in:Theoretical and applied genetics 1992-11, Vol.85 (2-3), p.229-238
Main Authors: Ayliffe, M.A. (Adelaide Univ. (Australia). Dept. of Genetics), Timmis, J.N
Format: Article
Language:English
Subjects:
ADN
Agriculture
Agronomy
Biological and medical sciences
CHLOROPLASTE
CHLOROPLASTS
CLOROPLASTO
DNA
DNA transfer
Fundamental and applied biological sciences. Psychology
GENE TRANSFER
Genes. Genome
Genetics & Heredity
Horticulture
Life Sciences & Biomedicine
Molecular and cellular biology
Molecular genetics
NICOTIANA TABACUM
Nuclear DNA
Plant Sciences
PLASTE
Plastid DNA
PLASTIDIOS
PLASTIDS
Promiscuous DNA
Science & Technology
TRANSFERENCIA DE GENES
TRANSFERT DE GENE
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Summary:Long tracts of DNA with high sequence homology to chloroplast DNA were isolated from nuclear genomic libraries of Nicotiana tabacum. One lambda EMBL4 clone was characterised in detail and assigned to nuclear DNA. The majority of the 15.5-kb sequence is greater than 99% homologous with its chloroplast DNA counterpart, but a single base deletion causes premature termination of the reading frame of the psaA gene. One region of the clone contains a concentration of deleted regions, and these were used to identify and quantify the sequence in native nuclear DNA by polymerase chain reaction (PCR) methods. An estimated 15 copies of this specific region are present in a lc tobacco nucleus.
ISSN:0040-5752
1432-2242
DOI:10.1007/BF00222864