Prolactin Synthesized and Secreted by Human Peripheral Blood Mononuclear Cells: An Autocrine Growth Factor for Lymphoproliferation

Prolactin has been shown to have an immunoregulatory role in the rodent immune response. A prolactin-like molecule has also been found in mouse splenocytes and a human B-lymphoblastoid cell line. We have evaluated whether human peripheral blood mononuclear cells (PBMCs) synthesize and/or secrete pro...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1992-08, Vol.89 (16), p.7713-7716
Main Authors: Sabharwal, P., Glaser, R., Lafuse, W., Varma, S., Liu, Q., Arkins, S., Kooijman, R., Kutz, L., Kelley, K. W., Malarkey, W. B.
Format: Article
Language:English
Subjects:
Analysis of the immune response. Humoral and cellular immunity
Antiserum
Base Sequence
Biological and medical sciences
Blood
Blotting, Southern
Blotting, Western
Cell Line
Cell lines
Cellular biology
Complementary DNA
DNA - blood
DNA - genetics
DNA - isolation & purification
DNA Replication
Enzyme-Linked Immunosorbent Assay
Exons
Female
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Gels
Growth Substances - biosynthesis
Hormones
Humans
Immune Sera
Immunobiology
Leukocytes, Mononuclear - physiology
Lymphatic system
Lymphocytes
Lymphocytes - immunology
Medical research
Messenger RNA
Miscellaneous
Molecular Sequence Data
Molecules
Multidisciplinary Sciences
Oligodeoxyribonucleotides
Placenta
Placenta - physiology
Polymerase chain reaction
Polymerase Chain Reaction - methods
Pregnancy
Prolactin - biosynthesis
Prolactin - blood
Prolactin - genetics
Regulatory factors and their cellular receptors
RNA, Messenger - analysis
RNA, Messenger - metabolism
Science & Technology
Science & Technology - Other Topics
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Summary:Prolactin has been shown to have an immunoregulatory role in the rodent immune response. A prolactin-like molecule has also been found in mouse splenocytes and a human B-lymphoblastoid cell line. We have evaluated whether human peripheral blood mononuclear cells (PBMCs) synthesize and/or secrete prolactin. We used the polymerase chain reaction (PCR) to generate a 276-base-pair prolactin product from human PBMCs, and Southern blot analysis confirmed that it was related to prolactin. Western blotting using a polyclonal antibody to prolactin indicated that cell extracts prepared from human PBMCs contained a high molecular mass (60-kDa) immunoreactive prolactin. To determine whether this PBMC prolactin was being secreted, we developed a highly sensitive and specific hormonal enzyme-linked immunoplaque assay. With this assay, we were able to detect human prolactin secretion from concanavalin A (Con A)- or phytohemagglutinin-stimulated PBMCs but not from unstimulated PBMCs. We next sought to determine whether this secreted prolactin could function as an autocrine growth factor in lymphoproliferation. We observed that anti-human prolactin antiserum significantly inhibited human PBMC proliferation in response to Con A or phytohemagglutinin. We conclude that a prolactin-like molecule is synthesized and secreted by human PBMCs and that it functions in an autocrine manner as a growth factor for lymphoproliferation.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.89.16.7713