Silver-enhanced radial immunodiffusion assay of plasma apolipoproteins

Silver-staining of immunoprecipitates extends the sensitivity of the radial immunodiffusion assay by tenfold. This modification permits the quantification of apolipoproteins A-I, A-II, C, and E at levels of 0.2-1.0 mg/dl in plasma samples at a sensitivity threshold of 10 ng. The silver-enhanced radi...

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Bibliographic Details
Published in:Journal of lipid research 1992-07, Vol.33 (7), p.1073-1078
Main Authors: Ishida, BY, Paigen, B
Format: Article
Language:English
Subjects:
Aminoacids, peptides. Hormones. Neuropeptides
Analytical, structural and metabolic biochemistry
Animals
Apolipoproteins - blood
Biochemistry & Molecular Biology
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Immunodiffusion - methods
Life Sciences & Biomedicine
Mice
Proteins
Science & Technology
Sensitivity and Specificity
Silver
Staining and Labeling
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Summary:Silver-staining of immunoprecipitates extends the sensitivity of the radial immunodiffusion assay by tenfold. This modification permits the quantification of apolipoproteins A-I, A-II, C, and E at levels of 0.2-1.0 mg/dl in plasma samples at a sensitivity threshold of 10 ng. The silver-enhanced radial immunodiffusion method is readily adapted from the standard method, simple and inexpensive to perform, and does not require costly instrumentation. These advantages make the modified RID assay an attractive alternative to other forms of immunoassay.
ISSN:0022-2275
1539-7262
DOI:10.1016/S0022-2275(20)41423-3