Nanogram level quantitation of oxycodone in human plasma by capillary gas chromatography using nitrogen—phosphorus selective detection

A sensitive and specific capillary gas chromatographic assay is reported for the quantitation of oxycodone in human plasma. The technique involves a single extraction of oxycodone and internal standard (hydrocodone) from plasma by toluene containing 1% isopropanol. Separation is achieved on a methyl...

Full description

Saved in:
Bibliographic Details
Published in:Journal of chromatography. Biomedical applications 1992-06, Vol.577 (2), p.283-287
Main Authors: Kapil, Ram P., Padovani, Patricia K., King, Shang-Ying P., Lam, Gilbert N.
Format: Article
Language:English
Subjects:
Analysis
Biological and medical sciences
Chemistry
Chemistry, Analytical
General pharmacology
Medical sciences
Pharmacology. Drug treatments
Physical Sciences
Science & Technology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A sensitive and specific capillary gas chromatographic assay is reported for the quantitation of oxycodone in human plasma. The technique involves a single extraction of oxycodone and internal standard (hydrocodone) from plasma by toluene containing 1% isopropanol. Separation is achieved on a methyl silicone (HP-1) fused-silica capillary column (25 m × 0.2 mm I.D., 0.33 μm film thickness) and detection is by nitrogen—phosphorus selective mode. The minimum quantifiable limit is 1.8 ng/ml using 2 ml of plasma. The method is applicable to characterize the plasma profile of oxycodone in humans after a single oral 5-mg oxycodone hydrochloride tablet.
ISSN:0378-4347
DOI:10.1016/0378-4347(92)80249-P