Pitfalls in the Double Labelling Immunoelectron Microscopy Using One Face of the Grid

We compared two procedures in the double labelling immunoelectron microscopic method of hormone-storing cells: one-faced and two-faced procedures. With the two-faced procedure we got convincing results consistent with morphological finding, while we often got false results with the one-faced procedu...

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Bibliographic Details
Published in:Journal of electron microscopy 1992-04, Vol.41 (2), p.120-122
Main Authors: Furuhata, Shigeru, Nemoto, Noriko, Miyazawa, Shichiro, Otani, Mitsuhiro, Toya, Shigeo, Kameya, Toru
Format: Article
Language:English
Subjects:
Amylases - analysis
Amylases - immunology
Biological and medical sciences
contamination
Diverse techniques
double labelling immunoelectron microscopy
Fundamental and applied biological sciences. Psychology
Growth Hormone - analysis
Growth Hormone - immunology
Humans
Immune Sera - analysis
Immune Sera - immunology
Immunohistochemistry
Microscopy
Microscopy, Immunoelectron - instrumentation
Microscopy, Immunoelectron - methods
Molecular and cellular biology
one-faced method
Pituitary Gland, Anterior - chemistry
Pituitary Gland, Anterior - cytology
Pituitary Gland, Anterior - ultrastructure
Prolactin - analysis
Prolactin - immunology
protein A gold
Science & Technology
Technology
two-faced method
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Summary:We compared two procedures in the double labelling immunoelectron microscopic method of hormone-storing cells: one-faced and two-faced procedures. With the two-faced procedure we got convincing results consistent with morphological finding, while we often got false results with the one-faced procedure. By the misleading one-faced procedure, the cell storing any one hormone will be misinterpreted as a multihormone storing cell. Therefore, it is concluded that the two-faced method is safer for the double labelling immunoelectron microscopic study.
ISSN:0022-0744
1477-9986
1477-9986
DOI:10.1093/oxfordjournals.jmicro.a050938