Cotyledons: An explant for routine regeneration of sunflower [Helianthus annuus] plants

The potential of plant regeneration from sunflower cotyledon explants was studied under conditions either to allow direct regeneration (organogenesis) or callus proliferation with high regeneration capacity. Analysis of histological modifications caused by tissue culture conditions showed that the r...

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Bibliographic Details
Published in:Plant and cell physiology 1992-03, Vol.33 (2), p.157-164
Main Authors: Ceriani, M.F. (Instituto de Biologia Molecular, Buenos Aires (Argentina)), Hopp, H.E, Hahne, G, Escandon, A.S
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cell Biology
COTILEDONES
COTYLEDON
COTYLEDONS
EXPLANT
EXPLANTES
EXPLANTS
Fundamental and applied biological sciences. Psychology
HELIANTHUS ANNUUS
Helianthus annuus L
Helianthus argophyllus
Life Sciences & Biomedicine
Plant physiology and development
Plant regeneration
Plant Sciences
REGENERACION
REGENERATION
Science & Technology
Tissue cultures, protoplasts
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Summary:The potential of plant regeneration from sunflower cotyledon explants was studied under conditions either to allow direct regeneration (organogenesis) or callus proliferation with high regeneration capacity. Analysis of histological modifications caused by tissue culture conditions showed that the regeneration protocol described here permitted the development of abundant meristematic centers in discrete regions of the basal part of the cotyledon. The protocol presented here proved to be suitable for the regeneration of 10 genotypes, among 20 assayed, including commercial inbred lines and hybrids of common use for production in Argentina. Furthermore, Agrobacterium infection experiments showed that basal halves of cotyledons were transformed, evidencing tumour proliferation.
ISSN:0032-0781
1471-9053
1471-9053
DOI:10.1093/oxfordjournals.pcp.a078234