Distribution of Adenosine-5’-Diphosphatase Activity in the Lactating Bovine Mammary Gland

A Ca2+- and Mg2+-stimulated adenosine-5’-diphosphatase has been found in lactating bovine mammary glands. The enzyme is associated with membranes of mitochondrial, microsomal, and Golgi apparatus fractions. The pH activity curves for the Golgi apparatus and microsomal fractions display two distinct...

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Bibliographic Details
Published in:Journal of dairy science 1992-03, Vol.75 (3), p.732-738
Main Authors: Basch, Jay J., Leung, Cecilia T., Wickham, Edward D., Farrell, Harold M.
Format: Article
Language:English
Subjects:
actividad enzimatica
activite enzymatique
adenosindifosfato
adenosine diphosphate
adenosine triphosphate
adenosine-5'-diphosphatase
adenosintrifosfato
Agriculture
Agriculture, Dairy & Animal Science
Animals
aparato golgi
appareil de golgi
Apyrase - analysis
Apyrase - antagonists & inhibitors
Apyrase - physiology
Biological and medical sciences
Calcium - pharmacology
casein
cattle
Cattle - physiology
citoplasma
cytoplasm
cytoplasme
cytoplasmic organelles
dairy cows
distribution
endoplasmic reticulum
Enzyme Activation
enzymes
enzymic activity
Female
Food industries
Food Science & Technology
Fundamental and applied biological sciences. Psychology
glande mammaire
glandulas mamarias
Golgi apparatus
Golgi Apparatus - enzymology
Hydrogen-Ion Concentration
Intracellular Membranes - enzymology
lactacion
lactation
Lactation - physiology
Life Sciences & Biomedicine
Magnesium - pharmacology
mammary gland
mammary glands
Mammary Glands, Animal - enzymology
Mammary Glands, Animal - ultrastructure
Microscopy, Electron
Microsomes - enzymology
milk
Milk and cheese industries. Ice creams
mitochondria
Mitochondria - enzymology
mitochondrie
mitocondria
nucleotidasa
nucleotidase
organite cellulaire
organulos citoplasmaticos
Science & Technology
secretion
vacas lecheras
vache laitiere
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Summary:A Ca2+- and Mg2+-stimulated adenosine-5’-diphosphatase has been found in lactating bovine mammary glands. The enzyme is associated with membranes of mitochondrial, microsomal, and Golgi apparatus fractions. The pH activity curves for the Golgi apparatus and microsomal fractions display two distinct maxima, one at pH 5.8 and the other between pH 7.4 and 8.4. Studies with activators and inhibitors indicate the enzyme is similar to adenosine-5’-diphosphatase found in other tissues. Its occurrence in the Golgi apparatus fraction indicates a possible role for this enzyme in the milk secretory process, particularly in ATP cycling in vesicles. Its occurrence in the microsomal fraction suggests a role in plasma membrane functioning.
ISSN:0022-0302
1525-3198
DOI:10.3168/jds.S0022-0302(92)77810-2