MYB56 Encoding a R2R3 MYB Transcription Factor Regulates Seed Size in Arabidopsis thaliana

Plant seed size is tightly regulated by the development of seed coat, embryo, and endosperm; however, currently, its underlying mechanism remains unclear. In this study, we revealed a regulatory role of an R2R3 MYB transcription factor MYB56 in controlling seed size specifically in Arabidopsis thali...

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Bibliographic Details
Published in:Journal of integrative plant biology 2013-11, Vol.55 (11), p.1166-1178
Main Authors: Zhang, Yanjie, Liang, Wanqi, Shi, Jianxin, Xu, Jie, Zhang, Dabing
Format: Article
Language:English
Subjects:
Arabidopsis - anatomy & histology
Arabidopsis - genetics
Arabidopsis - growth & development
Arabidopsis - metabolism
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Cell Proliferation
Cell wall
Cell Wall - genetics
Cotyledon - genetics
Cotyledon - growth & development
Endothelial Cells - cytology
endothelium
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
Gene Knockdown Techniques
Gene Knockout Techniques
Genes, Plant
MYB
MYB转录因子
seed coat
seed size
Seeds - anatomy & histology
Seeds - genetics
Seeds - growth & development
Seeds - metabolism
Time Factors
Transcription Factors - genetics
Transcription Factors - metabolism
发育过程
拟南芥属
种子大小
编码
血管内皮细胞
调控
过量表达
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Summary:Plant seed size is tightly regulated by the development of seed coat, embryo, and endosperm; however, currently, its underlying mechanism remains unclear. In this study, we revealed a regulatory role of an R2R3 MYB transcription factor MYB56 in controlling seed size specifically in Arabidopsis thaliana L. Loss- of-function or knock-down of MYB56 yielded smaller seeds as compared with the wild type. Conversely, overexpression of MYB56 produced larger seeds. Further observation using semi-thin sections showed that myb56 developed smaller contracted endothelial cells and reduced cell number in the outer integument layer of the seed coat during the seed development; by contrast, MYB56 overexpressing lines had expanded endothelial cells and increased cell number in the outer integument layer of the seed coat, suggesting the essential role of MYB56 in regulating seed development. In addition, reciprocal cross- analysis showed that MYB56 affected the seed development maternally. MYB56 was shown to be dominantly expressed in developing seeds, consistently with its function in seed development. Moreover, quantitative reverse transcription polymerase chain reaction analysis revealed that MYB56 regulates the expression of genes involved in cell wall metabolism such as cell division and expansion. Altogether, our results demonstrated that MYB56 represents an unknown pathway for positively controlling the seed size.
ISSN:1672-9072
1744-7909
DOI:10.1111/jipb.12094