Proliferating cell nuclear antigen involved in the repair process of ouabain-induced brain damage independent of hypertension in rats

Background Ouabain is a mammalian adrenocortical hormone that is involved in the pathogenesis of hypertension by inhibiting Na-K ATPase activity.It also participates in a variety of kinase-mediated signaling pathways associated with Na-K ATPase.Previous studies have shown that ouabain can cause card...

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Published in:Chinese medical journal 2014, Vol.127 (11), p.2111-2116
Main Authors: You, Beian, Shen, Lin, Qiu, Jie, Liu, Xiangju, Zhao, Shaohua, Ji, Xiang, Wang, Yan, Gao, Haiqing
Format: Article
Language:English
Subjects:
Animals
Blood Pressure - drug effects
Hypertension - drug therapy
Hypertension - metabolism
Immunohistochemistry
Male
Ouabain - therapeutic use
Proliferating Cell Nuclear Antigen - metabolism
Rats
Rats, Sprague-Dawley
Real-Time Polymerase Chain Reaction
SD大鼠
Sodium-Potassium-Exchanging ATPase - metabolism
Western印迹法
修复过程
哇巴因
增殖细胞核抗原
脑损伤
诱导
高血压
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Summary:Background Ouabain is a mammalian adrenocortical hormone that is involved in the pathogenesis of hypertension by inhibiting Na-K ATPase activity.It also participates in a variety of kinase-mediated signaling pathways associated with Na-K ATPase.Previous studies have shown that ouabain can cause cardiac remodeling independent of elevated blood pressure and that proliferating cell nuclear antigen （PCNA） plays a coordinating role for numerous proteins involved in multiple processes associated with DNA synthesis.Therefore,we hypothesized that ouabain might play a role in the cerebral cortex through signaling pathways independent of hypertension.And PCNA might be involved in this process.Methods Male Sprague-Dawley rats were treated with ouabain or with 0.9% nitric sodium as the control group.Systolic blood pressure was recorded weekly.After four weeks of treatment,morphological changes in the cerebral cortex were analyzed using light and transmission electron microscopy.The expression of PCNA in the cerebral cortex was evaluated by immunohistochemistry,real time quantitative PCR,and Western blotting.Results After 4-week treatment,there was no significant difference in systolic blood pressure compared with the control group,but both structural deterioration and up-regulated expression of PCNA in the brain was induced by ouabain treatment.Conclusions These results suggest that ouabain induces alterations in the brain structure,and this effect is independent of blood pressure.PCNA might be involved in the repair process of ouabain-induced brain damage.
ISSN:0366-6999
2542-5641
DOI:10.3760/cma.j.issn.0366-6999.20132286