Surveillance of Mycoplasma pneumoniae infection among children in Beijing from 2007 to 2012

Background Mycoplasma pneumonia （M.pneumoniae） is one of the key pathogens of community-acquired pneumonia.A global pandemic of M.pneumoniae has occurred since 2010.The aim of this study was to survey the prevalence of M.pneumoniae in children in Beijing from 2007-2012.Methods A total of 3 073 clini...

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Published in:Chinese medical journal 2014-04, Vol.127 (7), p.1244-1248
Main Authors: Zhao, Hanqing, Li, Shaoli, Cao, Ling, Yuan, Yi, Xue, Guanhua, Feng, Yanling, Yan, Chao, Wang, Liqiong, Fan, Zhaoyang, Sun, Hongmei
Format: Article
Language:English
Subjects:
Adolescent
Anti-Bacterial Agents - therapeutic use
Child
Child, Preschool
Drug Resistance, Bacterial
Female
GenBank
Humans
Macrolides - therapeutic use
Male
Mycoplasma pneumoniae - pathogenicity
Pneumonia, Mycoplasma - drug therapy
Pneumonia, Mycoplasma - epidemiology
Prevalence
儿童
北京
大环内酯类
巢式聚合酶链反应
支原体感染
琼脂糖凝胶电泳
肺炎支原体
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Summary:Background Mycoplasma pneumonia （M.pneumoniae） is one of the key pathogens of community-acquired pneumonia.A global pandemic of M.pneumoniae has occurred since 2010.The aim of this study was to survey the prevalence of M.pneumoniae in children in Beijing from 2007-2012.Methods A total of 3 073 clinical specimens were obtained from pediatric patients with respiratory tract infections from January 2007 to December 2012,and examined by nested polymerase chain reaction.PCR products were visualized by 2％ agarose gel electrophoresis,positive products sequenced,and compared with reference sequences in GenBank.Macrolide resistance-associated mutations were also detected for some positive samples.Results Of the 3 073 specimens,588 （19.13％） were positive for M.pneumoniae,12.4％ of which were accompanied by viral infections.Positive rates for M.pneumoniae were highest in 2007 and 2012,showing a significant difference when compared with other years.Infections tended to occur in autumn and winter and positive rates were significantly higher for children aged 3-16.The rate of macrolide resistance-associated mutations was 90.7％,and the predominant mutation was an A→G transition （89.92％） at position 2063 in domain V of the 23S rRNA gene.Conclusions M.pneumoniae outbreaks occurred in 2007 and 2012 in pediatric patients in Beijing,which is consistent with the global prevalence of M.pneumoniae.M.pneumoniae can cause multi-system infections in children,and may be accompanied with viral infections.We determined that school-age children are more susceptible to this disease,particularly in autumn and winter.Gene mutations associated with macrolide resistance were very common in M.pneumoniae-positive specimens during this period in Beijing.
ISSN:0366-6999
2542-5641
DOI:10.3760/cma.j.issn.0366-6999.20131654