Electrophysiological characteristics of cardiomyocyte-like cells from rat bone marrow derived mesenchymal stem cells by four inductors

Background Bone marrow derived mesenchymal stem cel induced by different inductors individually or collectively. In s （BMdMSCs） can differentiate into cardiomyocyte-like cells this study, by inducing BMdMSCs with p53 inhibitor （p-fifty three inhibitor-alpha, PFT-a）, 5-azacytidine （5-AZA）, angiotensi...

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Published in:Chinese medical journal 2013, Vol.126 (18), p.3528-3533
Main Authors: Liu, Bo-Wu, Lü, An-Lin, Hou, Jing, Huang, Wei, Hou, Hong, Hou, Zhao-Lei, DA, Jing, Ai, Shi-Yi
Format: Article
Language:English
Subjects:
Animals
Blotting, Western
Bone Marrow Cells - cytology
Cell Differentiation - physiology
Cells, Cultured
Electrophysiology - methods
Male
Mesenchymal Stromal Cells - cytology
Myocytes, Cardiac - cytology
Rats
Rats, Sprague-Dawley
大鼠
心肌样细胞
激光扫描共聚焦显微镜
电感器
电生理特性
血管紧张素II
骨形态发生蛋白-2
骨髓间充质干细胞
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Summary:Background Bone marrow derived mesenchymal stem cel induced by different inductors individually or collectively. In s （BMdMSCs） can differentiate into cardiomyocyte-like cells this study, by inducing BMdMSCs with p53 inhibitor （p-fifty three inhibitor-alpha, PFT-a）, 5-azacytidine （5-AZA）, angiotensin-II （Ang-II） and bone morphogenic protein-2 （BMP-2） we compared the influences of four inductors on the differentiation of rat BMdMSCs into caridomyocyte like-cells. Methods BMdMSCs were collected from the bone marrow of Sprague Dawley rats and after the fourth generation, the purified cells were divided into five groups： 5-AZA （10 μmol/L）, Ang-II （0.1 μmol/L）, PFT-α （20 μmol/L）, BMP-2 （10μg/L） and control. The purity of the BMdMSCs and the cardiac differentiation rates were obtained by flow cytometry. The expressions of cTnT in the BMdMSCs after four weeks of induction were detected by immunofluorescence and the expressions of cTnl and Cx43 detected by Western blotting. The green fluorescent levels reflecting intracellular calcium transient function were determined by laser scanning confocal microscopy. The total potassium current levels of cells were measured on patch clamp. Results All inductors affected to a different degree the differentiation of BMdMSCs into cardiomyocyte-like cells and the expressions of cTnT, cTnl and Cx43 suggesting that the combination of inductors could be an improved method for cardiac regenerative medicine. In addition, the total potassium current level and calcium transient in PFT-a cardiomyocyte-like cells were higher than other groups. Conclusions The cardiac differentiation of BMdMSCs induced by PFT-α, 5-AZA, Ang-II and BMP-2 has been improved at different levels. PFT-a has an advantage of differentiation rate and electrophysiological function over other inductors.
ISSN:0366-6999
2542-5641
DOI:10.3760/cma.j.issn.0366-6999.20122675