Reversal of multidrug resistance in renal cell carcinoma by short hairpin RNA targeting MDR1 gene

Background Over-expression of P-glycoprotein （P-gp）, encoded by the MDR1 gene, confers multidrug resistance （MDR） in renal cell carcinoma （RCC） and is a major reason for unsuccessful chemotherapy. This study aimed to determine the effct of RNA interference （RNAi） on the reversal of MDR in human RCC....

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Bibliographic Details
Published in:Chinese medical journal 2012-08, Vol.125 (15), p.2741-2745
Main Authors: Hao, Yi-Xin, He, Zheng-Wen, Zhu, Jian-Hua, Shen, Qian, Sun, Jun-Zhong, DU, Nan, Xiao, Wen-Hua
Format: Article
Language:English
Subjects:
ATP-Binding Cassette, Sub-Family B, Member 1 - genetics
ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism
Carcinoma, Renal Cell - genetics
Carcinoma, Renal Cell - metabolism
Cell Line, Tumor
Cell Proliferation
Humans
Inhibitory Concentration 50
Lentivirus - genetics
MDR1基因
mRNA表达水平
RNA, Small Interfering - genetics
RNA, Small Interfering - metabolism
RNA干扰
多药耐药
癌
短发夹RNA
肾细胞
逆转
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Summary:Background Over-expression of P-glycoprotein （P-gp）, encoded by the MDR1 gene, confers multidrug resistance （MDR） in renal cell carcinoma （RCC） and is a major reason for unsuccessful chemotherapy. This study aimed to determine the effct of RNA interference （RNAi） on the reversal of MDR in human RCC. Methods We designed and selected one short hairpin RNA （shRNA） targeting MDR1 gene, which is stably expressed from integrated plasmid and transfected by lentivirus fluid in human RCC A498 cell. Results The MDRl-targeted RNAi resulted in decreased MDR1 gene mRNA level （P 〈0.001）, almost abolished P-gp expression and reversed MDR to different chemotherapy drugs in the RCC A498 cell line. Conclusion MDR could be reversed by RNAi in human RCC A498 cell line, which may be used for clinical application
ISSN:0366-6999
2542-5641
DOI:10.3760/cma.j.issn.0366-6999.2012.15.016