Changes of histology and capsular collagen in a rat shoulder immobilization model

Shoulder immobilization can induce adhesion of the joint, capsular contracture or lead to the condition of frozen shoulder. However, little is known about the histological effects of immobilization on the shoulder joint. This study aimed to explore the effect of immobility on the subscapular bursa （...

Full description

Saved in:
Bibliographic Details
Published in:Chinese medical journal 2011-12, Vol.124 (23), p.3939-3944
Main Authors: Liu, Yu-Lei, Ao, Ying-Fang, Cui, Guo-Qing, Zhu, Jing-Xian
Format: Article
Language:English
Subjects:
Animals
Collagen - metabolism
Disease Models, Animal
Immunohistochemistry
Joint Capsule - metabolism
Male
Rats
Rats, Sprague-Dawley
Shoulder Dislocation - metabolism
Shoulder Dislocation - pathology
Shoulder Joint - metabolism
Shoulder Joint - pathology
免疫组化检测
固定模
国家统计局
类型分布
组织学观察
老鼠
肩关节
胶原蛋白
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Shoulder immobilization can induce adhesion of the joint, capsular contracture or lead to the condition of frozen shoulder. However, little is known about the histological effects of immobilization on the shoulder joint. This study aimed to explore the effect of immobility on the subscapular bursa （SSB） and the joint capsular content, including the distribution of typesⅠ and Ⅲcollagen, within an immobilized rat shoulder. Methods Forty-six Sprague-Dawley rats were randomly divided into one control group （n=6） and four immobilization groups （n=10 in each group）, in which the left shoulders were immobilized with plaster for 1,2, 3 and 4 weeks. At the end of each time point, 2 rats from each group were euthanized and shoulders prepared for serial histological observations of the glenohumeral joints, as well as picrosirius red and immunohistochemical observation of type Ⅲcollagen. Histological sections of the remaining rat shoulders were used for the immunohistochemical detection of the capsular content of types Ⅰand Ⅲcollagen. Results The hyperplastic synovium of the anterior capsule obstructed the communication between the SSB and the glenohumeral joint cavity at 2 and 3 weeks. The adhesion of the SSB appeared at 3 and 4 weeks. The quantitative and qualitative results showed that the capsular contents of types Ⅰand Ⅲcollagen progressively increased at 2, 3 and 4 weeks, and that type Ⅲcollagen was distributed extensively within the joint capsule at 2 and 3 weeks. Conclusion Immobilization of the rat shoulder induced synovial hyperplasia of the joint capsule, adhesion of the SSB and an increase of the capsular content of types Ⅰ and Ⅲcollagen.
ISSN:0366-6999
2542-5641
DOI:10.3760/cma.j.issn.0366-6999.2011.23.019