Altered nuclear factor-kappaB inducing kinase expression in insulin-resistant mice

Background Insulin resistance is an underlying feature of both type 2 diabetes and metabolic syndrome. Currently, it is unclear whether nuclear factor （NF）-KB inducing kinase （NIK） plays a role in the development of insulin resistance. The present in vivo study investigated the roles of NIK and IKB...

Full description

Saved in:
Bibliographic Details
Published in:Chinese medical journal 2011-11, Vol.124 (22), p.3646-3651
Main Authors: Su, Lei, Xiu, Ling-Ling, Wei, Guo-Hong, Zhong, Xing, Liu, Yuan-Yuan, Cao, Xiao-Pei, Li, Yan-Bing, Xiao, Hai-Peng
Format: Article
Language:English
Subjects:
Animals
Blotting, Western
Body Weight - physiology
Fasting - blood
Glucose Tolerance Test
I-kappa B Kinase - metabolism
IKB激酶
Insulin - blood
Insulin Resistance - physiology
Kidney - metabolism
Liver - metabolism
Male
Mice
Mice, Inbred C57BL
Mice, Obese
NF-kappaB-Inducing Kinase
Protein Serine-Threonine Kinases - metabolism
Western印迹
二甲双胍
小鼠
核因子-κB
胰岛素增敏剂
胰岛素抵抗
诱导
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background Insulin resistance is an underlying feature of both type 2 diabetes and metabolic syndrome. Currently, it is unclear whether nuclear factor （NF）-KB inducing kinase （NIK） plays a role in the development of insulin resistance. The present in vivo study investigated the roles of NIK and IKB kinase a （IKKa） in obesity-induced insulin resistance using animal models. Methods NIK expression was evaluated by Western blotting in male Lepob mice and C57BL/6J mice fed a high-fat diet （HFD） （45% fat）. After metformin and sulfasalazine treatment, NIK expression was investigated during the improvement of insulin resistance. Results NIK was increased by about 1-fold in the renal tissues of Lepab mice and C57BL/6J mice fed a HFD for 12 weeks. After 1 and 3 weeks of high-fat feeding, we observed an almost 50% decrease in NIK and IKKa expression in the liver and renal tissues of C57BL/6J mice. NIK expression was significantly lower in the liver and renal tissues of HFD-fed mice that were treated with insulin sensitizers, metformin and sulfasalazine. However, IKKa expression was increased after metformin treatment in both tissues. Conclusion These results suggest a possible role of NIK in the liver and renal tissues of insulin-resistant mice.
ISSN:0366-6999
2542-5641
DOI:10.3760/cma.j.issn.0366-6999.2011.22.009