Reduced brain-derived neurotrophic factor expression in cortex and hippocampus involved in the learning and memory deficit in molarless SAMP8 mice

Background The molarless condition has been reported to compromise learning and memory functions. However, it remains unclear how the molarless condition directly affects the central nervous system, and the functional consequences on the brain cortex and hippocampus have not been described in detail...

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Bibliographic Details
Published in:Chinese medical journal 2011-05, Vol.124 (10), p.1540-1544
Main Authors: Jiang, Qing-Song, Liang, Zi-Liang, Wu, Min-Jie, Feng, Lin, Liu, Li-Li, Zhang, Jian-Jun
Format: Article
Language:English
Subjects:
Animals
Blotting, Western
Body Weight - physiology
Brain-Derived Neurotrophic Factor - metabolism
Cerebral Cortex - metabolism
Hippocampus - metabolism
Male
Maze Learning
Memory Disorders - metabolism
Mice
Motor Activity - physiology
Protein-Tyrosine Kinases - metabolism
学习能力
小鼠
海马
皮层
老年痴呆症
脑源性神经营养因子
记忆体
高亲和力受体
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Summary:Background The molarless condition has been reported to compromise learning and memory functions. However, it remains unclear how the molarless condition directly affects the central nervous system, and the functional consequences on the brain cortex and hippocampus have not been described in detail. The aim of this study was to find the molecular mechanism related with learning and memory deficit after a bilateral molarless condition having been surgically induced in senescence-accelerated mice/prone8 （SAMP8） mice, which may ultimately provide an experimental basis for clinical prevention of senile dementia.Methods Mice were either sham-operated or subjected to complete molar removal. The animals＇ body weights were monitored every day. Learning ability and memory were measured in a water maze test at the end of the 1 st, 2nd, and 3rd months after surgery. As soon as significantly prolonged escape latency in the molarless group was detected, the locomotor activity was examined in an open field test. Subsequently, the animals were decapitated and the cortex and hippocampus were dissected for Western blotting to measure the expression levels of brain-derived neurotrophic factor （BDNF） and the tropomyosin related kinase B （TrkB）, the high affinity receptor of BDNF.Results Slightly lower weights were consistently observed in the molarless group, but there was no significant difference in weights between the two groups （P〉0.05）. Compared with the sham group, the molarless group exhibited lengthened escape latency in the water maze test three months after surgery, whereas no difference in locomotor activity was observed. Meanwhile, in the cortex and hippocampus, BDNF levels were significantly decreased in the molarless group （P〈0.05）; but the expression of its receptor, TrkB, was not significantly affected.Conclusion These results suggested that the molarless condition impaired learning and memory abilities in SAMP8mice three months after teeth extraction, and this effect was accompanied by significantly reduced BDNF expression in the cortex and hippocampus.
ISSN:0366-6999
2542-5641
DOI:10.3760/cma.j.issn.0366-6999.2011.10.019