Inducible nitric oxide synthase is involved in the oxidation stress induced by HIV-1 gp120 in human retina pigment epithelial cells

Background The human immunodeficiency virus-1 （HIV-1） envelope glycoprotein gp120 has been implicated in the development of AIDS-associated retinopathy. The present study tested the hypothesis that gp120 may induce oxidative stress including up regulation of inducible nitric oxide synthase （iNOS） an...

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Published in:Chinese medical journal 2008-12, Vol.121 (24), p.2578-2583
Main Authors: Yu, Qiu-rong, Zhang, Zhen-ping, Zhang, Hui, Lin, Hao-tian, Li, Xiu-mei, Bai, Ling, Cai, Wei-bin
Format: Article
Language:English
Subjects:
Blotting, Western
Cell Line
Epithelial Cells - drug effects
Epithelial Cells - metabolism
gp120
HIV Envelope Protein gp120 - pharmacology
HIV-1
Humans
Immunohistochemistry
Microscopy, Confocal
Nitric Oxide - metabolism
Nitric Oxide Synthase Type II - genetics
Nitric Oxide Synthase Type II - metabolism
Oxidative Stress - drug effects
Polymerase Chain Reaction
Retina - cytology
色素上皮细胞
视网膜
诱导性氧化氮合酶
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Summary:Background The human immunodeficiency virus-1 （HIV-1） envelope glycoprotein gp120 has been implicated in the development of AIDS-associated retinopathy. The present study tested the hypothesis that gp120 may induce oxidative stress including up regulation of inducible nitric oxide synthase （iNOS） and production of malondialdehyde （MDA） and nitric oxide （NO） to mediate retinopathy in retinal pigment epithelial （RPE） cells. Methods Human RPE cell line D407 was cultured and treated with gp120. HIV-1 gp120 protein induced lipid peroxidation product MDA. NO production and iNOS expression were examined in vitro by spectrophomtometry, real-time PCR, Western blotting, and confocal microscope. Results Addition of gp120 was able to induce RPE cells to produce NO and MDA in time- and dose-dependent manners （P 〈0.05）. Similarly, gp120 was also capable of up-regulating iNOS mRNA and protein in D407 cells in time- and dose-dependent manners. Conclusions Gp120 induces oxidative stress in D407 cell by stimulating MDA and NO production, which is mediated by up-regulating iNOS expression. Gp120 may mediate oxidation stress in AIDS-associated retinopathy.
ISSN:0366-6999
2542-5641
DOI:10.1097/00029330-200812020-00020