Rapid HPLC Method for Determination of 12 Isoflavone Components in Soybean Seeds

S1; It is important to determine the isoflavone components by high-performance liquid chromatography(HPLC)for the molecular assistant selection of isoflavone in soybean.Based on the standard samples of 12 isoflavone components,the isoflavone components were analyzed using the determination of absorb...

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Bibliographic Details
Published in:中国农业科学（英文版） 2011, Vol.10 (1), p.70-77
Main Authors: SUN Jun-ming, SUN Bao-li, HAN Fen-xia, YAN Shu-rong, YANG Hua, Akio Kikuchi
Format: Article
Language:English
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Summary:S1; It is important to determine the isoflavone components by high-performance liquid chromatography(HPLC)for the molecular assistant selection of isoflavone in soybean.Based on the standard samples of 12 isoflavone components,the isoflavone components were analyzed using the determination of absorbance peaks method by HPLC.The results showed that there were different maximum ultraviolet(UV)absorbance for the aglycones of daidzein,glycitein,and genistein,which were at 250,257,and 260 nm,respectively.A linear gradient elution of acetonitrile(13-30%)containing 0.1% acetic acid as a mobile phase was applied on a YMC-C18 column at 35℃.The 12 isoflavone components were determined using the UV detector by HPLC.We concluded that this is a rapid and precise method which adapted to determine the large numbers of samples with microanalysis.
ISSN:1671-2927
DOI:10.1016/S1671-2927(09)60291-1