A novel aptasensor strategy for protein detection based on G-quadruplex and exonuclease III-aided recycling amplification

Herein we developed a sensitive and selective fluorescent aptasensor for target detection, integrating the excellent specificity between the aptamer and target, high sensitivity of the fluorescence signal provided by G-quadruplex and Exo III-aided recycling amplification. [Display omitted] The detec...

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Bibliographic Details
Published in:Chinese chemical letters 2020-01, Vol.31 (1), p.155-158
Main Authors: Shi, Huan, Jin, Tian, Zhang, Jiewen, Huang, Xiaoting, Tan, Chunyan, Jiang, Yuyang, Tan, Ying
Format: Article
Language:English
Subjects:
Aptasensor
Exonuclease III-aided recycling amplification
G-quadruplex
Magnetic beads
MUC1
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Summary:Herein we developed a sensitive and selective fluorescent aptasensor for target detection, integrating the excellent specificity between the aptamer and target, high sensitivity of the fluorescence signal provided by G-quadruplex and Exo III-aided recycling amplification. [Display omitted] The detection of biomarkers is of great significance in the diagnosis of numerous diseases, especially cancer. Herein, we developed a sensitive and universal fluorescent aptasensor strategy based on magnetic beads, DNA G-quadruplex, and exonuclease III (Exo III). In the presence of a target protein, a label-free single strand DNA (ssDNA) hybridized with the aptamer was released as a trigger DNA due to specific recognition between the aptamer and target. Subsequently, ssDNA initiates the Exo III-aided recycling to amplify the fluorescence signal, which was caused by N-methylmesoporphyrin IX (NMM) insertion into the G-quadruplex structure. This proposed strategy combines the excellent specificity between the aptamer and target, high sensitivity of the fluorescence signal by G-quadruplex and Exo III-aided recycling amplification. We selected (50–1200 nmol/L) MUC1, a common tumor biomarker, as the proof-of-concept target to test the specificity of our aptasensor. Results reveal that the sensor sensitively and selectively detected the target protein with limits of detection (LODs) of 3.68 and 12.83 nmol/L in buffer solution and 10% serum system, respectively. The strategy can be easily applied to other targets by simply substituting corresponding aptamers and has great potential in the diagnosis and monitoring of several diseases.
ISSN:1001-8417
1878-5964
DOI:10.1016/j.cclet.2019.06.020