Identification of target genes of transcription factor CEBPB in acute promyelocytic leukemia cells induced by all-trans retinoic acid

<正>Objective:To indentify target genes of transcription factor CCA AT enhancer-binding protein P(CEBPB) in acute proinyelocytie leukemia cells induced by all-tram retinoie acid.Methods: A new strategy for high—throughput identification of direct target genes was established by combinin...

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Bibliographic Details
Published in:Asian Pacific journal of tropical medicine 2013-06, Vol.6 (6), p.473-480
Main Authors: Yu, Lei, Zhang, Yang-De, Zhou, Jun, Yao, De-Ming, Li, Xiang
Format: Article
Language:English
Subjects:
acid
All-trans
All-trans retinoic acid
Binding Sites
CCAAT-Enhancer-Binding Protein-beta - genetics
CCAAT-Enhancer-Binding Protein-beta - metabolism
CEBP
Cell Differentiation - drug effects
Cell Differentiation - genetics
Cell Line
Chromatin
Chromatin Immunoprecipitation
Electrophoretic Mobility Shift Assay
Gene Expression Regulation, Neoplastic
Granulopoiesis
Humans
immunoprecipitation
Leukemia, Promyelocytic, Acute - genetics
Leukemia, Promyelocytic, Acute - metabolism
Neutrophils
Neutrophils - drug effects
Neutrophils - physiology
Polymerase Chain Reaction
retinoic
Signal Transduction - drug effects
Tretinoin - pharmacology
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Summary:<正>Objective:To indentify target genes of transcription factor CCA AT enhancer-binding protein P(CEBPB) in acute proinyelocytie leukemia cells induced by all-tram retinoie acid.Methods: A new strategy for high—throughput identification of direct target genes was established by combining chromatin immunoprecipitation(ChIP) with in vitro selection.Then,106 potential CKBPB binding fragments from the genome of the all-trans retinoie acid(ATRA)-treated NB4 cells were identified.Results:Of them,82 were mapped in proximity to known or previously predicted genes;7 were randomly picked up for further confirmation by ChlP-PCR and 3 genes (CALM,1TPR2 and 0RM2) were found to be specificaUy up-regulated in the ATRA-treated NB4 cells,indicating that they might lie the down-stream target genes of ATKA.Conclusions:Our results provided new insight into the mechanisms of ATRA-induced granulocytic differentiation.
ISSN:1995-7645
2352-4146
DOI:10.1016/S1995-7645(13)60077-2