Anti-oxidant and anti-inflammatory activity of leaf extracts and fractions of Mangifera indica

<正>Objective:To evaluate the anti-oxidani and anti-inflammatory activity of leaf extracts and fractions of Mangifera indica in in vitro conditions.Methods:In vitro DPPH radical scavenging activity and lipoxygenase（LOX） inhibition assays were used to evaluate the anti-oxidant and anti-i...

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Published in:Asian Pacific journal of tropical medicine 2013-04, Vol.6 (4), p.311-314
Main Authors: Mohan, CG, Deepak, M, Viswanatha, GL, Savinay, G, Hanumantharaju, V, Rajendra, CE, Halemani, Praveen D
Format: Article
Language:English
Subjects:
Anti-inflammatory
Anti-Inflammatory Agents - chemistry
Antioxidants
Antioxidants - chemistry
assay
Biphenyl Compounds - chemistry
DPPH
DPPH assay
indica
Lipoxygenase
Lipoxygenase - analysis
Lipoxygenase Inhibitors - chemistry
Mangifera
Mangifera indica
Picrates - chemistry
Plant Extracts - chemistry
Plant Leaves
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Summary:<正>Objective:To evaluate the anti-oxidani and anti-inflammatory activity of leaf extracts and fractions of Mangifera indica in in vitro conditions.Methods:In vitro DPPH radical scavenging activity and lipoxygenase（LOX） inhibition assays were used to evaluate the anti-oxidant and anti-inflammatory activities respectively.Methanolic extract（MEMI）,successive water extract （SWMI） and ethyl acetate fraction（EMEMI）,n-butanol fraction（BMEMI） and water soluble fraction （WMEMI） of methanolic extract were evaluated along with respective reference standards. Results:In in ritro DPPH radical scavenging activity,the MEMI,EMEMI and BMEMI have offered significant antioxidant activity with IC50 values of 13.37.3.55 and 14.19μig/mL respectively.Gallic acid,a reference standard showed significant antioxidant activity with IC? value of 1.88 and found to be more potent compared to all the extracts and fractions.In m vitro LOX inhibition assay,the MEMI,EMEMI and BMEMI have showed significant inhibition of LOX enzyme activity with IC50 values of 96.71.63.21 and 107.44μg/mL respectively.While,reference drug Indometlhacin also offered significant inhibtion against LOX enzyme activity with IC50 of 57.75.Furthermore,MEMI was found to more potent than SWMI and among the fractions EMEMI was found to possess more potent antioxidant and anti-inflammatory activity.Conclusions:These findings suggest that the MEMI and F.MEMT possess potent anti-oxidani and anti-inflammatory activities in in vitro conditions.
ISSN:1995-7645
2352-4146
DOI:10.1016/S1995-7645(13)60062-0