Chromosome analysis of esophageal squamous cell carcinoma cell line KYSE 410-4 by repetitive multicolor fluorescence in situ hybridization

Chromosome aberrations are distinctive features of human malignant tumors. Analysis of chromosomal changes can illuminate the molecular mechanisms underlying the development and progression of cancer. To establish the technique of multicolor fluorescence in situ hybridization （M-FISH） for identifyin...

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Bibliographic Details
Published in:Journal of genetics and genomics 2008, Vol.35 (1), p.11-16
Main Authors: Yang, Yiling, Chu, Jiayou, Wu, Yupeng, Luo, Manli, Xu, Xin, Han, Yaling, Cai, Yan, Zhan, Qimin, Wang, Mingrong
Format: Article
Language:chi ; eng
Subjects:
410-4
Carcinoma, Squamous Cell - genetics
Carcinoma, Squamous Cell - pathology
Cell Line, Tumor
Chromosome Aberrations
Chromosome Banding
Chromosome Deletion
Color
Esophageal Neoplasms - genetics
Esophageal Neoplasms - pathology
esophageal squamous cell carcinoma
Humans
In Situ Hybridization, Fluorescence - methods
karyotype
Karyotyping
KYSE
KYSE 410-4
Male
Metaphase - genetics
Middle Aged
multicolor fluorescence in situ hybridization
多色荧光杂交
染色体分析
核型
食道鳞状细胞癌
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Summary:Chromosome aberrations are distinctive features of human malignant tumors. Analysis of chromosomal changes can illuminate the molecular mechanisms underlying the development and progression of cancer. To establish the technique of multicolor fluorescence in situ hybridization （M-FISH） for identifying chromosome aberrations in esophageal carcinoma cell line KYSE 410-4, four pools of 6-color whole-chromosome painting probes have been designed and hybridized on the same metaphase spread by four rounds of repetitive FISH. Repetitive 6-color M-FISH was successfully established and the cytogenetic abnormalities in KYSE 410-4 cells were characterized. Chromosome gains occurred at 2q, 3, 8, 17p, and X. An isochromosome 3q was visualized in the cell line, which might be one intermediate mechanism leading to 3p losses and/or 3q gains. Furthermore, 16 structural arrangements were detected, including four derivative chromosomes. The rearrangement of the centromeric regions accounted for approximately 44% of all rearrangements. The results added a more complete and accurate information of the genetic alterations to the classical cytogenetic description of KYSE 410-4 and provided a detailed cytogenetic background data for appropriate use of the cell line. The established 6-color M-FISH was useful for analyzing chromosomes in the whole genome of human tumors.
ISSN:1673-8527
DOI:10.1016/S1673-8527(08)60002-8