Difference in hTERT Gene Expressions between HbsAg-Positive and HbsAg-Negative Hepatocellular Carcinoma

To investigate the difference in expression of hTERT gene between HbsAg-positive human hepatocellular carcinoma (HCC) and HbsAg-negative HCC and to explore the relationship be tween HBV infection and hTERT gene expression in HCC. The expression of hTERT protein in 30 cases of HbsAg positive HCCC and...

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Bibliographic Details
Published in:Current medical science 2005, Vol.25 (3), p.303-306
Main Author: 郭悦青 周旭 刘恩宇 李兴睿 刘谨文 杨志芳 易继林
Format: Article
Language:English
Subjects:
Adult
Aged
Carcinoma, Hepatocellular - enzymology
Carcinoma, Hepatocellular - virology
Female
HbsAg
Hepatitis B - immunology
Hepatitis B Surface Antigens - blood
hTERT
Humans
Liver Neoplasms - enzymology
Liver Neoplasms - virology
Male
Middle Aged
RNA, Messenger - biosynthesis
RNA, Messenger - genetics
Telomerase - biosynthesis
Telomerase - genetics
乙型肝炎病毒
基因表达
肝细胞癌
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Summary:To investigate the difference in expression of hTERT gene between HbsAg-positive human hepatocellular carcinoma (HCC) and HbsAg-negative HCC and to explore the relationship be tween HBV infection and hTERT gene expression in HCC. The expression of hTERT protein in 30 cases of HbsAg positive HCCC and 17 cases of HbsAg negative HCC was detected by immunohistochemistry (SP method), and the expression of hTERT mRNA was analyzed by reverse transcription polymerase chain reaction (RT-PCR). t-test, Chi-squared test and cochran- armitage trend test were used to see whether there was an interrelation between HBsAg and hTERT gene in HCC. The expression of hTERT protein was mostly located in plasm and occasionally in the nucleus of liver cancer cells, The positive rate of hTERT protein and hTERT mRNA in HbsAg positive HCC- 93.33% (28/a0) and 83. 33% (25/30) respectively which were much higher than those in HbsAg negative HCC- 52.94% (9/17), 47.06% (8/17) (P
ISSN:1672-0733
2096-5230
1993-1352
2523-899X
DOI:10.1007/bf02828150