Analysis of bacterial community in bulking sludge using culture-dependent and -independent approaches

The bacterial community of a bulking sludge from a municipal wastewater treatment plant with anoxic-anaerobic-oxic process was investigated by combination of cultivation and 16S rRNA gene clone library analysis for understanding the causes of bulking.A total of 28 species were obtained from 63 isola...

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Bibliographic Details
Published in:Journal of environmental sciences (China) 2011-01, Vol.23 (11), p.1880-1887
Main Authors: Jin, Decai, Wang, Ping, Bai, Zhihui, Wang, Xinxin, Peng, Hong, Qi, Rong, Yu, Zhisheng, Zhuang, Guoqiang
Format: Article
Language:English
Subjects:
16S rRNA gene clone library
Acinetobacter
Aeromonas
Bacteria - classification
Bacteria - genetics
Bacteria - growth & development
bacterial communities
Banding
beta-Proteobacteria
Biodiversity
bulking sludge
cultivable bacteria
culture media
Culture Techniques
DNA Fingerprinting
DNA primers
Gene Library
genes
Genes, Bacterial
Genes, rRNA
Klebsiella
Microbial Consortia
municipal wastewater
Polymerase Chain Reaction
Pseudomonas
REP
rep-PCR
ribosomal RNA
RNA, Ribosomal, 16S - genetics
rRNA基因
sequence analysis
Sewage - microbiology
sludge
wastewater treatment
城市污水处理厂
微生物群落
文化
污泥膨胀
细菌群落
聚合酶链反应
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Summary:The bacterial community of a bulking sludge from a municipal wastewater treatment plant with anoxic-anaerobic-oxic process was investigated by combination of cultivation and 16S rRNA gene clone library analysis for understanding the causes of bulking.A total of 28 species were obtained from 63 isolates collected from six culture media.The most cultivable species belonged to γ-Proteobacteria including Klebsiella sp.,Pseudomonas sp.,Aeromonas sp.and Acinetobacter sp.Further analysis of these strains by repetitive sequence based on polymerase chain reaction （rep-PCR） technology showed that rep-PCR yielded discriminatory banding patterns within the same genus using REP and BOX primer sets.While the culture-independent assessment revealed that β-Proteobacteria was the dominant group in the bulking sample.Sequence analysis revealed that the highest proportion （14.7%） of operational taxonomic units was 98% similar to Candidatus Accumulibacter phosphatis,which is used to remove phosphorous from wastewater.Our results indicated that combining different approaches can produce complementary information,thus generate a more accurate view of microbial community in bulking sludge.
ISSN:1001-0742
1878-7320
DOI:10.1016/S1001-0742(10)60621-3