Gold Nanoparticles Based Colorimetric Detection of Target DNA After Loop-mediated Isothermal Amplification

We have developed a rapid, simple and label-free colorimetric method for the identification of target DNA It is based on loop-mediated isothermal amplification（LAMP）. Plain gold nanoparticles（AuNPs） are used to indicate the occurrence of LAME The amplified product is mixed with AuNPs in an optimized...

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Bibliographic Details
Published in:Chemical research in Chinese universities 2013-06, Vol.29 (3), p.424-428
Main Authors: Zhou, Chao, Mu, Ying, Yang, Meng-chao, Wu, Qing-qing, Xu, Wei, Zhang, Ying, Jin, Wei, Song, Qi, Wu, Zhong-yu, Jin, Qin-han
Format: Article
Language:English
Subjects:
Analytical Chemistry
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
DNA鉴定
dNTPs浓度
Inorganic Chemistry
Organic Chemistry
Physical Chemistry
介导
比色法
等温
纳米金
脱氧核糖核苷酸
金纳米粒子
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Summary:We have developed a rapid, simple and label-free colorimetric method for the identification of target DNA It is based on loop-mediated isothermal amplification（LAMP）. Plain gold nanoparticles（AuNPs） are used to indicate the occurrence of LAME The amplified product is mixed with AuNPs in an optimized ratio, at which the deoxyribonucleotides（dNTPs） bind to the AuNPs via ligand-metal interactions and thus enhance AuNPs stability. If a target DNA is amplified, the dramatic reduction of the dNTPs leads to the aggregation of AuNPs and a color change from red to blue. The success of the method strongly depends on the ionic strength of the solution and the initial concentration of dNTPs. Unlike other methods for the identification of isothermal products, this method is simple and can be readily applied on site where instrumentation is inadequate or even lacking.
ISSN:1005-9040
2210-3171
DOI:10.1007/s40242-013-2371-9