Synthesis of Vitamin E Succinate from Candida rugosa Lipase in Organic Medium

A screening of commercially available lipases for the synthesis of vitamin E succinate showed that lipase from Candida rugosa presented the highest yield. The synthesis of vitamin E succinate in organic solvents with dif- ferent lgP values ranging from -1.3 to 3.5 was investigated. Of particular int...

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Bibliographic Details
Published in:Chemical research in Chinese universities 2013-04, Vol.29 (2), p.223-226
Main Authors: Jiang, Xiang-jun, Hu, Yi, Jiang, Ling, Gong, Ji-hong, Huang, He
Format: Article
Language:English
Subjects:
Analytical Chemistry
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
DMSO
Inorganic Chemistry
Organic Chemistry
Physical Chemistry
假丝酵母
合成
有机介质
有机溶剂
生物相容性
维生素E琥珀酸酯
酵母脂肪酶
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Summary:A screening of commercially available lipases for the synthesis of vitamin E succinate showed that lipase from Candida rugosa presented the highest yield. The synthesis of vitamin E succinate in organic solvents with dif- ferent lgP values ranging from -1.3 to 3.5 was investigated. Of particular interest was that dimethyl sulfoxide （DMSO） with the lowest lgP exhibited the highest yield among all the organic solvents used. It suggests that lgP is incapable of satisfactorily predicting the biocompatibility of organic solvents due to the complexity of enzymatic reaction with hydrophilic and hydrophobic substrates in organic solvent. Effects of different operating conditions, such as molar ratio of substrate, enzyme concentration, reaction temperature, mass transfer, and reaction time were also studied. Under the optimum conditions of 10 g/L enzyme, a stirring rate of 100 r/min, a substrate molar ratio of 5：1 at 55℃ for 18 h, a satisfactory yield（46.95%） was obtained. The developed method has a potential to be used for efficient enzymatic production of vitamin E succinate.
ISSN:1005-9040
2210-3171
DOI:10.1007/s40242-013-2486-z