miR-93-5p Transferred by Exosomes Promotes the Proliferation of Esophageal Cancer Cells via Intercellular Communication by Targeting PTEN

To investigate the relationship between plasma miR-93-5p and the risk of esophageal cancer, as well as the influence of miR-93-5p on the biological function of esophageal cancer cells, exerted through exosomes. The expression of plasma miR-93-5p in esophageal cancer patients and healthy controls was...

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Bibliographic Details
Published in:Biomedical and environmental sciences 2018-03, Vol.31 (3), p.171-185
Main Authors: LIU, Meng Xin, LIAO, Juan, XIE, Ming, GAO, Zhi Kui, WANG, Xiang Hu, ZHANG, Ying, SHANG, Mu He, YIN, Li Hong, PU, Yue Pu, LIU, Ran
Format: Article
Language:English
Subjects:
Esophageal cancer
Exosomes
miR-93-5p
Plasma biomarker
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Summary:To investigate the relationship between plasma miR-93-5p and the risk of esophageal cancer, as well as the influence of miR-93-5p on the biological function of esophageal cancer cells, exerted through exosomes. The expression of plasma miR-93-5p in esophageal cancer patients and healthy controls was analysed by real-time quantitative PCR. The influence of miR-93-5p on the risk and prognosis of esophageal carcinoma was analyzed by conditional logistic regression and survival analysis. The effect of miR-93-5p on the biological function of recipient cells was investigated by establishing an in vitro donor cell co-culture model. The target gene of miR-93-5p was validated by luciferase reporter assay and Western Blotting. Upregulation of plasma miR-93-5p expression significantly increases the risk of esophageal cancer and is associated with poor prognosis. miR-93-5p transferred by exosomes promotes the proliferation of recipient esophageal cancer cells and affects the expression of PTEN and its downstream proteins p21 and cyclin D1. Our study provides a reference for the identification of biomarkers for the diagnosis and prognosis of esophageal cancer.
ISSN:0895-3988
2214-0190
DOI:10.3967/bes2018.023