A New Method for Blood NT-proBNP Determination Based on a Near-infrared Point of Care Testing Device with High Sensitivity and Wide Scope

Objective To develop a rapid, highly sensitive, and quantitative method for the detection of NT-proBNP levels based on a near-infrared point-of-care diagnostic （POCT） device with wide scope. Methods The lateral flow assay （LFA） strip of NT-proBNP was first prepared to achieve rapid detection. Then,...

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Bibliographic Details
Published in:Biomedical and environmental sciences 2017-06, Vol.30 (6), p.426-431
Main Authors: ZHANG, Xiao Guang, SHU, Yao Gen, GAO, Ju, WANG, Xuan, LIU, Li Peng, WANG, Meng, CAO, Yu Xi, ZENG, Yi
Format: Article
Language:English
Subjects:
Antibodies
Biomarkers
CT装置
Heart Diseases - diagnosis
Humans
Immunoassay - methods
Infrared Rays
Lateral flow assay
Natriuretic Peptide, Brain - blood
Near-infrared POCT device
NT-proBNP
Peptide Fragments - blood
Point-of-Care Testing
Reagent Strips
Sensitivity and Specificity
临床验证
外点
宽范围
检测仪
测定
硝酸纤维素膜
高灵敏度
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Summary:Objective To develop a rapid, highly sensitive, and quantitative method for the detection of NT-proBNP levels based on a near-infrared point-of-care diagnostic （POCT） device with wide scope. Methods The lateral flow assay （LFA） strip of NT-proBNP was first prepared to achieve rapid detection. Then, the antibody pairs for NT-proBNP were screened and labeled with the near-infrared fluorescent dye Dylight-800. The capture antibody was fixed on a nitrocellulose membrane by a scribing device. Serial dilutions of serum samples were prepared using NT-proBNP-free serum series. The prepared test strips, combined with a near-infrared POCT device, were validated by known concentrations of clinical samples. The POCT device gave the output of the ratio of the intensity of the fluorescence signal of the detection line to that of the quality control line. The relationship between the ratio value and the concentration of the specimen was plotted as a work curve. The results of 62 clinical specimens obtained from our method were compared in parallel with those obtained from the Roche E411 kit. Results Based on the log-log plot, the new method demonstrated that there was a good linear relationship between the ratio value and NT-proBNP concentrations ranging from 20 pg/mL to 10 ng/mL. The results of the 62 clinical specimens measured by our method showed a good linear correlation with those measured by the Roche E411 kit. Conclusion The new LFA detection method of NT-proBNP levels based on the near-infrared POCT device was rapid and highly sensitive with wide scope and was thus suitable for rapid and early clinical diagnosis of cardiac impairment.
ISSN:0895-3988
2214-0190
DOI:10.3967/bes2017.056