H5N1 Avian Influenza Pre-pandemic Vaccine Strains in China

Objective To prepare the 4 candidate vaccine strains of H5N1 avian influenza virus isolated in China Methods Recombinant viruses were rescued using reverse genetics. Neuraminidase （NA） and hemagglutinin （HA） segments of the A/Xinjiang/1/2006, A/Guangxi/1/2009, A/Hubei/1/2010, and A/Guangdong/1/2011...

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Published in:Biomedical and environmental sciences 2014-10, Vol.27 (10), p.763-769
Main Authors: BO, Hong, Bo DONG, Li, ZHANG, Ye, DONG, Jie, Mei ZOU, Shu, Bao GAO, Rong, Yan WANG, Da, Long SHU, Yue
Format: Article
Language:English
Subjects:
Animals
Chick Embryo
Chickens
China
H5N1
H5N1禽流感病毒
Hemagglutinin Glycoproteins, Influenza Virus - genetics
Hemagglutinin Glycoproteins, Influenza Virus - metabolism
Influenza
Influenza A Virus, H5N1 Subtype - immunology
Influenza in Birds - prevention & control
Influenza in Birds - virology
Influenza Vaccines - immunology
Neuraminidase - genetics
Neuraminidase - metabolism
Pre-pandemic
Reverse Transcriptase Polymerase Chain Reaction
Vaccine strains
Vaccines, Synthetic - immunology
中国
低致病性禽流感
反向遗传学
疫苗株
神经氨酸酶
胰蛋白酶
重组病毒
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Summary:Objective To prepare the 4 candidate vaccine strains of H5N1 avian influenza virus isolated in China Methods Recombinant viruses were rescued using reverse genetics. Neuraminidase （NA） and hemagglutinin （HA） segments of the A/Xinjiang/1/2006, A/Guangxi/1/2009, A/Hubei/1/2010, and A/Guangdong/1/2011 viruses were amplified by RT-PCR. Multibasic amino acid cleavage site of HA was removed and ligated into the pCIpoll vector for virus rescue. The recombinant viruses were evaluated by trypsin dependent assays. Their embnjonate survival and antigenicity were compared with those of the respective wild-type viruses. Results The 4 recombinant viruses showed similar antigenicity compared with wild-type viruses, chicken embryo survival and trypsin-dependent characteristics. Conclusion The 4 recombinant viruses rescued using reverse genetics meet the criteria for classification of low pathogenic avian influenza strains, thus supporting the use of them for the development of seeds and production of pre-pandemic vaccines.
ISSN:0895-3988
2214-0190
DOI:10.3967/bes2014.111