ROOT ULTRAVIOLET B-SENSITIVE1/WEAK AUXIN RESPONSE3 Is Essential for Polar Auxin Transport in Arabidopsis

The phytohormone auxin regulates virtually every aspect of plant development. To identify new genes involved in auxin activity, a genetic screen was performed for Arabidopsis (Arabidopsis thaliana) mutants with altered expression of the auxinresponsive reporter DR5rev:GFP. One of the mutants recover...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 2013-06, Vol.162 (2), p.965-976
Main Authors: Yu, Hong, Karampelias, Michael, Robert, Stephanie, Peer, Wendy Ann, Swarup, Ranjan, Ye, Songqing, Ge, Lei, Cohen, Jerry, Murphy, Angus, Friml, Jirí, Estelle, Mark
Format: Article
Language:English
Subjects:
2,4-Dichlorophenoxyacetic Acid - pharmacology
Arabidopsis - drug effects
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Auxins
Biological and medical sciences
Biological Transport - genetics
Botanik
Botany
Cell Membrane - metabolism
Cell membranes
Endosomes - metabolism
F-Box Proteins - genetics
F-Box Proteins - metabolism
Fundamental and applied biological sciences. Psychology
Genetic mutation
Indoleacetic Acids - metabolism
Insulin antibodies
Membrane Transport Proteins - genetics
Membrane Transport Proteins - metabolism
MEMBRANES, TRANSPORT, AND BIOENERGETICS
Meristem - genetics
Meristem - metabolism
Mutation
Plant cells
Plant physiology and development
Plant roots
Plant Roots - genetics
Plant Roots - growth & development
Plants
Plants, Genetically Modified
Receptors, Cell Surface - genetics
Receptors, Cell Surface - metabolism
Root growth
Root tips
Seedlings
Signal Transduction
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Summary:The phytohormone auxin regulates virtually every aspect of plant development. To identify new genes involved in auxin activity, a genetic screen was performed for Arabidopsis (Arabidopsis thaliana) mutants with altered expression of the auxinresponsive reporter DR5rev:GFP. One of the mutants recovered in the screen, designated as weak auxin response3 (wxr3), much lower DR5rev:GFP expression when treated with the synthetic auxin 2,4-dichlorophenoxyacetic acid and displays severe defects in root development. The wxr3 mutant decreases polar auxin transport and results in a disruption of the asymmetric auxin distribution. The levels of the auxin transporters AUXIN1 and PIN-FORMED are dramatically reduced in the wxr3 root tip. Molecular analyses demonstrate that WXR3 is ROOT ULTRAVIOLET B-SENSITIVE1 (RUS1), a member of the conserved Domain of Unknown Function647 protein family found in diverse eukaryotic organisms. Our data suggest that RUS1/WXR3 plays an essential role in the regulation of polar auxin transport by maintaining the proper level of auxin transporters on the plasma membrane.
ISSN:0032-0889
1532-2548
1532-2548
DOI:10.1104/pp.113.217018