Combining transcriptomics and genetic linkage based information to identify candidate genes associated with Heterobasidion-resistance in Norway spruce

The Heterobasidion annosum s.l species complex comprises the most damaging forest pathogens to Norway spruce. We revisited previously identified Quantitative Trait Loci (QTLs) related to Heterobasidion-resistance in Norway spruce to identify candidate genes associated with these QTLs. We identified...

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Bibliographic Details
Published in:Scientific reports 2020-07, Vol.10 (1), p.12711-12711, Article 12711
Main Authors: Chaudhary, Rajiv, Lundén, Karl, Dalman, Kerstin, Dubey, Mukesh, Nemesio-Gorriz, Miguel, Karlsson, Bo, Stenlid, Jan, Elfstrand, Malin
Format: Article
Language:English
Subjects:
Ammonia
Basidiomycota - pathogenicity
Caffeoyl-CoA O-methyltransferase
Cinnamoyl-CoA reductase
Disease Resistance
Flavonoids
Flavonoids - biosynthesis
Flavonoids - metabolism
Gene expression
Gene Expression Profiling - methods
Gene Expression Regulation, Plant
Genes
Genetic analysis
Genetic Linkage
Genetics and Breeding
Genetik och förädling
High-Throughput Nucleotide Sequencing
Inoculation
Methyltransferase
Phenylalanine
Phenylalanine ammonia-lyase
Picea - genetics
Picea - microbiology
Picea abies
Plant Biotechnology
Plant Proteins - genetics
Quantitative Trait Loci
Sequence Analysis, RNA
Transcription factors
Transcription Factors - genetics
Trees
Växtbioteknologi
Wounding
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Summary:The Heterobasidion annosum s.l species complex comprises the most damaging forest pathogens to Norway spruce. We revisited previously identified Quantitative Trait Loci (QTLs) related to Heterobasidion-resistance in Norway spruce to identify candidate genes associated with these QTLs. We identified 329 candidate genes associated with the resistance QTLs using a gene-based composite map for Pinaceae. To evaluate the transcriptional responses of these candidate genes to H. parviporum, we inoculated Norway spruce plants and sequenced the transcriptome of the interaction at 3 and 7 days post inoculation. Out of 298 expressed candidate genes 124 were differentially expressed between inoculation and wounding control treatment. Interestingly, PaNAC04 and two of its paralogs in the subgroup III-3 of the NAC family transcription factors were found to be associated with one of the QTLs and was also highly induced in response to H. parviporum. These genes are possibly involved in the regulation of biosynthesis of flavonoid compounds. Furthermore, several of the differentially expressed candidate genes were associated with the phenylpropanoid pathway including a phenylalanine ammonia-lyase, a cinnamoyl-CoA reductase, a caffeoyl-CoA O-methyltransferase and a PgMYB11-like transcription factor gene. Combining transcriptome and genetic linkage analyses can help identifying candidate genes for functional studies and molecular breeding in non-model species.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-020-69386-0