Chemical Screening Pipeline for Identification of Specific Plant Autophagy Modulators

Autophagy is a major catabolic process in eukaryotes with a key role in homeostasis, programmed cell death, and aging. In plants, autophagy is also known to regulate agronomically important traits such as stress resistance, longevity, vegetative biomass, and seed yield. Despite its significance, the...

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Published in:Plant physiology (Bethesda) 2019-11, Vol.181 (3), p.855-866
Main Authors: Dauphinee, Adrian N, Cardoso, Catarina, Dalman, Kerstin, Ohlsson, Jonas A, Fick, Stina Berglund, Robert, Stéphanie, Hicks, Glenn R, Bozhkov, Peter V, Minina, Elena A
Format: Article
Language:English
Subjects:
Arabidopsis - cytology
Arabidopsis - drug effects
Autophagy - drug effects
Biochemistry and Molecular Biology
Biokemi och molekylärbiologi
Botanik
Botany
Cell Survival - drug effects
Drug Evaluation, Preclinical - methods
Macrolides - pharmacology
Morpholines - pharmacology
Nicotiana - cytology
Nicotiana - drug effects
Organic Chemicals - pharmacology
Thiadiazoles - pharmacology
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Summary:Autophagy is a major catabolic process in eukaryotes with a key role in homeostasis, programmed cell death, and aging. In plants, autophagy is also known to regulate agronomically important traits such as stress resistance, longevity, vegetative biomass, and seed yield. Despite its significance, there is still a shortage of reliable tools modulating plant autophagy. Here, we describe the first robust pipeline for identification of specific plant autophagy-modulating compounds. Our screening protocol comprises four phases: (1) high-throughput screening of chemical compounds in cell cultures of tobacco ( ); (2) confirmation of the identified hits in planta using Arabidopsis ( ); (3) further characterization of the effect using conventional molecular biology methods; and (4) verification of chemical specificity on autophagy in planta. The methods detailed here streamline the identification of specific plant autophagy modulators and aid in unraveling the molecular mechanisms of plant autophagy.
ISSN:0032-0889
1532-2548
1532-2548
DOI:10.1104/pp.19.00647