The Role of Lipopolysaccharide and Shiga-like Toxin in a Mouse Model of Escherichia coli O157:H7 Infection

The Role of Lipopolysaccharide and Shiga-like Toxin in a Mouse Model of Escherichia coli O157:H7 Infection

The role of lipopolysaccharide (LPS) and Shiga-like toxin (SLT) in the pathogenesis of hemolytic uremic syndrome (HUS) was studied in a mouse model. Mice inoculated intragastrically with Escherichia coli O157:H7 developed gastrointestinal, neurologic, and systemic symptoms, necrotic foci in the colo...

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Bibliographic Details
Published in:The Journal of infectious diseases 1997-03, Vol.175 (3), p.611-620
Main Authors: Karpman, Diana, Connell, Hugh, Svensson, Majlis, Scheutz, Flemming, Aim, Per, Svanborg, Catharina
Format: Article
Language:eng
Subjects:
Animal infectious diseases
Animals
Bacterial Toxins
Bacterial Toxins - toxicity
Basic Medicine
Biological and medical sciences
Blood
Disease Models, Animal
Escherichia coli
Escherichia coli Infections
Escherichia coli Infections - physiopathology
Escherichia coli O157
Escherichia coli O157 - pathogenicity
Female
General aspects
Hemolysis
Hemolytic uremic syndrome
Hemolytic-Uremic Syndrome - microbiology
Immunologi inom det medicinska området
Immunology in the medical area
Infections
Infectious diseases
Inoculation
Kidneys
Lipopolysaccharides
Lipopolysaccharides - toxicity
Major Articles
Male
Medical and Health Sciences
Medical sciences
Medicin och hälsovetenskap
Medicinska och farmaceutiska grundvetenskaper
Mice
Mice, Inbred C3H
Nervous system diseases
Pathology
Research Support, Non-U.S. Gov't
Shiga Toxin 2
Shiga toxins
Symptoms
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Summary:The role of lipopolysaccharide (LPS) and Shiga-like toxin (SLT) in the pathogenesis of hemolytic uremic syndrome (HUS) was studied in a mouse model. Mice inoculated intragastrically with Escherichia coli O157:H7 developed gastrointestinal, neurologic, and systemic symptoms, necrotic foci in the colon, glomerular and tubular histopathology, and fragmented erythrocytes. LPS-responder (C3H1HeN) mice developed a combination of neurologic and systemic symptoms, whereas LPS-nonresponder (C3H/HeJ) mice had a biphasic course of disease, first developing systemic symptoms and later severe neurologic symptoms. Mice inoculated with SLT-II-positive strains developed severe neurotoxic symptoms and a higher frequency of systemic symptoms and glomerular pathology compared with SLT-II-negative strains. Anti-SLT-II antibodies protected against these symptoms and pathology. These results demonstrate that this model could be used to study aspects of human HUS and that both LPS and SLT are important for disease development.
ISSN:0022-1899
1537-6613