miR-877-3p targets Smad7 and is associated with myofibroblast differentiation and bleomycin-induced lung fibrosis

Myofibroblast differentiation of lung resident mesenchymal stem cells (LR-MSC) plays an important role in idiopathic pulmonary fibrosis. By comparing the expression profiles of miRNAs before and after myofibroblast differentiation of LR-MSC, we identified miR-877-3p as a fibrosis-related miRNA. We f...

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Bibliographic Details
Published in:	Scientific reports 2016-07, Vol.6 (1), p.30122-30122, Article 30122
Main Authors:	Wang, Cong, Gu, Shen, Cao, Honghui, Li, Zutong, Xiang, Zou, Hu, Kebin, Han, Xiaodong
Format:	Article
Language:	eng
Subjects:	Animals Biological Sciences Biologiska vetenskaper Bleomycin - pharmacology Cell Differentiation - drug effects Cell Differentiation - physiology Fibroblasts - drug effects Fibroblasts - metabolism Fibroblasts - physiology Idiopathic Pulmonary Fibrosis - chemically induced Idiopathic Pulmonary Fibrosis - metabolism Idiopathic Pulmonary Fibrosis - physiopathology idiopathic pulmonary-fibrosis Lung - drug effects Lung - metabolism Male mesenchymal stem-cells Mesenchymal Stromal Cells - metabolism Mice Mice, Inbred C57BL micrornas MicroRNAs - metabolism Myofibroblasts - metabolism Myofibroblasts - physiology receptor Science & Technology - Other Topics Signal Transduction - physiology Smad7 Protein - metabolism tgf-beta therapy Transforming Growth Factor beta1 - metabolism Up-Regulation - physiology
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Summary:	Myofibroblast differentiation of lung resident mesenchymal stem cells (LR-MSC) plays an important role in idiopathic pulmonary fibrosis. By comparing the expression profiles of miRNAs before and after myofibroblast differentiation of LR-MSC, we identified miR-877-3p as a fibrosis-related miRNA. We found that miR-877-3p sequestration inhibited the myofibroblast differentiation of LR-MSC and attenuates bleomycin-induced lung fibrosis by targeting Smad7. Smad7, as an inhibitory smad in the TGF-β1 signaling pathway, was decreased in the myofibroblast differentiation of LR-MSC and up-regulation of Smad7 could inhibit the differentiation process. Our data implicates a potential application of miR-877-3p as a fibrosis suppressor for pulmonary fibrosis therapy and also as a fibrosis marker for predicting prognosis.
ISSN:	2045-2322 2045-2322