Lactobacilli from human gastrointestinal mucosa are strong stimulators of IL‐12 production

Interaction of macrophages with bacteria is a stimulus for production of cytokines such as IL‐10 and IL‐12. IL‐12 stimulates T cell and natural killer (NK) cell cytotoxicity and interferon‐gamma (IFN‐γ) production. IL‐10 opposes the T cell‐stimulating action of IL‐12, decreases the release of proinf...

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Bibliographic Details
Published in:	Clinical and experimental immunology 1999-05, Vol.116 (2), p.276-282
Main Authors:	HESSLE, C, HANSON, L. A, WOLD, A. E
Format:	Article
Language:	eng
Subjects:	Analysis of the immune response. Humoral and cellular immunity Biological and medical sciences biosynthesis Cells Cells, Cultured Cultured cytokines Escherichia coli Escherichia coli - physiology Fundamental and applied biological sciences. Psychology Fundamental immunology Gastric Mucosa Gastric Mucosa - microbiology Humans IL‐10 IL‐12 Immunobiology Immunologi inom det medicinska området Immunology in the medical area Interleukin-10 Interleukin-10 - biosynthesis Interleukin-12 Interleukin-12 - biosynthesis Intestinal Mucosa Intestinal Mucosa - microbiology Lactobacillus Lactobacillus - physiology Lactobacillus paracasei paracasei Lactobacillus plantarum Lactobacillus rhamnosus Leukocytes Leukocytes, Mononuclear - metabolism Lymphokines, interleukins ( function, expression) metabolism microbiology Microbiology in the medical area Mikrobiologi inom det medicinska området Monocytes Monocytes - metabolism Mononuclear Original physiology Regulatory factors and their cellular receptors
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Summary:	Interaction of macrophages with bacteria is a stimulus for production of cytokines such as IL‐10 and IL‐12. IL‐12 stimulates T cell and natural killer (NK) cell cytotoxicity and interferon‐gamma (IFN‐γ) production. IL‐10 opposes the T cell‐stimulating action of IL‐12, decreases the release of proinflammatory cytokines from macrophages, and stimulates B cells. We have studied the capacity of human intestinal isolates from the three Lactobacillus species dominating on the human gastrointestinal mucosa, L. plantarum, L. rhamnosus and L. paracasei ssp. paracasei, to induce production of IL‐10 and IL‐12 from human blood mononuclear cells, or monocytes. Whole killed lactobacilli were potent stimulators of IL‐12 over a wide range of bacterial concentrations. Lactobacillus paracasei gave the highest levels of IL‐12 (1.5 ng/ml in response to 5 × 106 bacteria/ml), roughly 10 times more than obtained by stimulation with L. rhamnosus or L. plantarum. Escherichia coli induced on average
ISSN:	0009-9104 1365-2249