Bacterial contaminants and antibacterial activity of honeys from Bunut Hilir and Jongkong districts, Kapuas Hulu Regency, West Borneo, Indonesia

This study aimed to investigate bacterial contaminants and antimicrobial activity of forest honey from Bunut Hilir (B) and Jongkong (J) districts, Kapuas Hulu regency, West Borneo, Indonesia. Identification of bacteria was done based on 16S rDNA sequencing. Antibacterial tests were carried out again...

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Bibliographic Details
Main Authors: Julistiono, Heddy, Arisandi, Pujawati, Mulyadi, Setyaningrum, Ninu, Noviana, Zahra, Dinoto, Achmad
Format: Conference Proceeding
Language:English
Subjects:
Antiinfectives and antibacterials
Bacteria
Coliforms
Contaminants
E coli
Honey
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Summary:This study aimed to investigate bacterial contaminants and antimicrobial activity of forest honey from Bunut Hilir (B) and Jongkong (J) districts, Kapuas Hulu regency, West Borneo, Indonesia. Identification of bacteria was done based on 16S rDNA sequencing. Antibacterial tests were carried out against Escherichia coli, Mycobacterium smegmatis, Staphylococcus aureus and contaminant bacteria using MTT assay method. The results showed that two and one bacteria isolates were found in honey B and J respectively. Isolate B1, B2 and J1 were close to Micrococcus luteus, Acinetobacter schindleri, and Micrococcus endophyticus, respectively. Both kinds of honey have antibacterial activity against target bacteria with a range of Minimum Inhibition Concentration (MIC) from 12.5 % (v/v) to above 25 % (v/v). The highest antibacterial activity of honey B was against S. aureus with a MIC of 12.5% (v/v). The lowest activity of honey B was in E. coli with a MIC of 25% (v/v). The highest activity of honey J was against S. aureus and isolate J1 with MIC value of 15% (v/v). The lowest activity of honey J was on Escherichia coli and isolate B1 with a MIC>25% (v/v). In E. coli and M. smegmatis, honey in low concentration (3%, v/v) caused an increase in the population that exceeds the negative control with 0% honey concentration. The data indicated that although the antibacterial activity of honey, in some circumstances bacterial cells can survive in honey.
ISSN:0094-243X
1551-7616
DOI:10.1063/5.0209392