Growth kinetics, effect of carbon substrate in biosynthesis of mcl-PHA by Pseudomonas putida Bet001

Growth associated biosynthesis of medium chain length poly-3-hydroxyalkanoates (mcl-PHA) in Pseudomonas putida Bet001 isolated from palm oil mill effluent was studied. Models with substrate inhibition terms described well the kinetics of its growth. Selected fatty acids (C8:0 to C18:1) and ammonium...

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Bibliographic Details
Published in:Brazilian journal of microbiology 2014-04, Vol.45 (2), p.427-438
Main Authors: Gumel, A M, Annuar, M S M, Heidelberg, T
Format: Article
Language:eng ; por
Subjects:
Ammonium Compounds - metabolism
Biosynthesis
Carbon
Carbon - metabolism
Culture Media - chemistry
Environmental Microbiology
Fatty Acids - metabolism
Industrial Microbiology
Industrial Waste
Kinetics
MICROBIOLOGY
Oils & fats
Polyhydroxyalkanoates - metabolism
Pseudomonas putida - growth & development
Pseudomonas putida - isolation & purification
Pseudomonas putida - metabolism
Substrates
Temperature
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Summary:Growth associated biosynthesis of medium chain length poly-3-hydroxyalkanoates (mcl-PHA) in Pseudomonas putida Bet001 isolated from palm oil mill effluent was studied. Models with substrate inhibition terms described well the kinetics of its growth. Selected fatty acids (C8:0 to C18:1) and ammonium were used as carbon and nitrogen sources during growth and PHA biosynthesis, resulting in PHA accumulation of about 50 to 69% (w/w) and PHA yields ranging from 10.12 g L(-1) to 15.45 g L(-1), respectively. The monomer composition of the PHA ranges from C4 to C14, and was strongly influenced by the type of carbon substrate fed. Interestingly, an odd carbon chain length (C7) monomer was also detected when C18:1 was fed. Polymer showed melting temperature (T m) of 42.0 (± 0.2) °C, glass transition temperature (T g) of -1.0 (± 0.2) °C and endothermic melting enthalpy of fusion (ΔHf) of 110.3 (± 0.1) J g(-1). The molecular weight (M w) range of the polymer was relatively narrow between 55 to 77 kDa.
ISSN:1517-8382
1678-4405
1678-4405
DOI:10.1590/S1517-83822014000200009