Transcriptional profiling of genes involved in n-hexadecane compounds assimilation in the hydrocarbon degrading Dietzia cinnamea P4 strain

The petroleum-derived degrading Dietzia cinnamea strain P4 recently had its genome sequenced and annotated. This allowed employing the data on genes that are involved in the degradation of n-alkanes. To examine the physiological behavior of strain P4 in the presence of n-alkanes, the strain was grow...

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Bibliographic Details
Published in:Brazilian journal of microbiology 2013-01, Vol.44 (2), p.633-647
Main Authors: Procópio, Luciano, de Cassia Pereira e Silva, Michele, van Elsas, Jan Dirk, Seldin, Lucy
Format: Article
Language:eng ; por
Subjects:
Actinomycetales - genetics
Actinomycetales - growth & development
Actinomycetales - metabolism
Alkanes - metabolism
Biodegradation
Biotransformation
Gene Expression Profiling
Genes
Genes, Bacterial
Hydrocarbons
Hydrocarbons - metabolism
Hydrogen-Ion Concentration
Metabolic Networks and Pathways - genetics
MICROBIOLOGY
Real-Time Polymerase Chain Reaction
Research Paper
Strain rate
Temperature
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Summary:The petroleum-derived degrading Dietzia cinnamea strain P4 recently had its genome sequenced and annotated. This allowed employing the data on genes that are involved in the degradation of n-alkanes. To examine the physiological behavior of strain P4 in the presence of n-alkanes, the strain was grown under varying conditions of pH and temperature. D. cinnamea P4 was able to grow at pH 7.0-9.0 and at temperatures ranging from 35 ºC to 45 ºC. Experiments of gene expression by real-time quantitative RT-PCR throughout the complete growth cycle clearly indicated the induction of the regulatory gene alkU (TetR family) during early growth. During the logarithmic phase, a large increase in transcriptional levels of a lipid transporter gene was noted. Also, the expression of a gene that encodes the protein fused rubredoxin-alkane monooxygenase was enhanced. Both genes are probably under the influence of the AlkU regulator.
ISSN:1517-8382
1678-4405
1678-4405
DOI:10.1590/S1517-83822013000200044