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In situ estimation of indigestible compounds contents in cattle feed and feces using bags made from different textiles
The objective of this experiment was to evaluate estimates of concentration of indigestible dry matter (iDM), indigestible neutral detergent fiber (iNDF) and indigestible acid detergent fiber (iADF) in samples of cattle feed and feces processed at different particle sizes (1-, 2-, and 3-mm) using ba...
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| Published in: | Revista brasileira de zootecnia 2011-03, Vol.40 (3), p.666-675 |
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| Main Authors: | , , , , , |
| Format: | Article |
| Language: | eng ; por |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
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| Summary: | The objective of this experiment was to evaluate estimates of concentration of indigestible dry matter (iDM), indigestible neutral detergent fiber (iNDF) and indigestible acid detergent fiber (iADF) in samples of cattle feed and feces processed at different particle sizes (1-, 2-, and 3-mm) using bags made from nylon (50 μm), F57 (Ankom®), and non-woven textile (NWT - 100 g/m²) in a in situ evaluation procedure. Eight samples of concentrates, seven samples of forages and two samples of cattle feces were used. The samples were put in the bags according to a ratio of 20 mg of dry matter/cm² of surface by using three bags of each textile for each size of particle, totaling 405 bags, which were submitted to ruminal incubation for 264 hours. The contents of indigestible compounds in the samples were evaluated sequentially. Blank bags were used for microscopic evaluation of textile physical integrity and for evaluation of the physical resistance. The presence of contaminants caused high variability of results of iDM, indicating that its using as internal marker should not be recommended. By using the textiles F57 and NWT, similar estimates for contents of iNDF and iADF were obtained whereas by using nylon (50 μm), the concentration of iNDF was underestimated, possibly because of loss of particles due to its porosity. Nylon presented loss of resistance in all analytical stages, expanding its probality of rupture, especially after extraction with acid detergent. For in situ evaluation of indigestible compounds, it is suggested grinding samples in 2-mm screen sieves.
Objetivou-se avaliar as estimativas de concentração de matéria seca (MSi), fibra em detergente neutro (FDNi) e fibra em detergente ácido (FDAi) indigestíveis em amostras de alimentos e fezes bovinas processadas em diferentes tamanhos de partículas (1, 2 e 3 mm) utilizando-se sacos confeccionados com os tecidos nylon (50 μm), F57 (Ankom®) e tecido não-tecido (TNT - 100 g/m²) em procedimento de avaliação in situ. Foram utilizadas oito amostras de alimentos concentrados, sete de alimentos volumosos e duas de fezes bovinas. O material foi acondicionado nos sacos numa relação de 20 mg de matéria seca/cm² de superfície utilizando-se três sacos de cada tecido para cada tamanho de partícula, totalizando 405 sacos, que foram submetidos a incubação ruminal durante 264 horas. Os teores dos compostos indigestíveis nas amostras foram avaliados sequencialmente. Sacos em branco foram utilizados para avaliação microsc |
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| ISSN: | 1516-3598 1806-9290 1516-3598 |
| DOI: | 10.1590/S1516-35982011000300027 |