Can Triamcinolone acetonide, platelet-rich plasma, and pentosan polysulfate sodium induce oxidative stress in cultured equine chondrocytes?

Progressive deterioration and loss of articular cartilage are the final degenerative events common to osteoarthritis (OA). Reactive oxygen species (ROS) play an important role in this chondrocyte catabolic activity, leading to cell death and matrix components breakdown. Intra-articular corticosteroi...

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Bibliographic Details
Published in:Ciência rural 2019, Vol.49 (7)
Main Authors: Palma, Heloisa Einloft, Gallio, Miguel, Silva, Gabriele Biavaschi da, Cantarelli, Camila, Wolkmer, Patrícia, Salazar, Rodrigo Fernando dos Santos, Wergutz, Julien, Krause, Luciana Maria Fontanari, Krause, Alexandre, Brass, Karin Erica, Corte, Flavio Desessards De La
Format: Article
Language:eng ; por
Subjects:
AGRONOMY
antioxidant enzymes
Antioxidants
Apoptosis
Arthritis
Biomedical materials
Blood platelets
Cartilage
Cartilage (articular)
Cartilage diseases
catalase
Cell culture
Cell death
Chondrocytes
Cytokines
Disease
Extracellular matrix
glutathione peroxidase
Laboratories
lipid peroxidation
Osteoarthritis
Oxidative stress
Pain
Pathogenesis
Pentosan polysulfate
Plasma
Platelets
Reactive oxygen species
Sodium
Triamcinolone acetonide
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Summary:Progressive deterioration and loss of articular cartilage are the final degenerative events common to osteoarthritis (OA). Reactive oxygen species (ROS) play an important role in this chondrocyte catabolic activity, leading to cell death and matrix components breakdown. Intra-articular corticosteroid injections such as triamcinolone acetonide have been used to control pain and inflammation associated with OA. New treatments for OA, platelet-rich plasma and pentosan polysulphate sodium have also been used and further investigations are necessary to determine their safety in joint cells. In this in vitro study, the use of these three substances (triamcinolone acetonide, platelet-rich plasma, and pentosan polysulphate sodium) in healthy chondrocytes did not alter the antioxidant status when compared to control groups, indicating that they could be considered safe in healthy conditions. RESUMO: A deterioração progressiva e perda da cartilagem articular são os eventos finais da osteoartrite (OA). Espécies reativas de oxigênio (ROS) têm papel importante na atividade catabólica de condrócitos, levando a morte celular e quebra dos componentes da matriz. Injeções intra-articulares de corticosteroides, como com o acetonido de triancinolona, são usadas para controle da dor e inflamação associadas à OA. Novos tratamentos para a OA, como o plasma rico em plaquetas e o pentosano polissulfato sódico, também tem sido utilizados e necessitam de maiores investigações para determinar sua segurança para as células articulares de equinos. Neste estudo in vitro, o uso destas três substâncias (acetonido de triancinolona, pentosan polissulfato de sódio de plasma rico em plaquetas) em condrócitos saudáveis de equinos não alterou o status antioxidante quando comparado aos grupos controle, indicando que puderam ser considerados seguros em condições saudáveis.
ISSN:0103-8478
1678-4596
1678-4596
DOI:10.1590/0103-8478cr20180263