High molecular mass fraction in clinical isolates of Paracoccidioides brasiliensis

Different serum levels of the IgG/IgE for Paracoccidioides brasiliensis high mass molecular (hMM) fraction (~366 kDa) in the acute and chronic forms of the disease have been reported. Considering the nonexistence of hMM fraction investigation involving clinical isolates of P. brasiliensis, the prese...

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Published in:Revista da Sociedade Brasileira de Medicina Tropical 2010-09, Vol.43 (5), p.526-530
Main Authors: Fredrich, Andréa Longoni, Nagashima, Luciene Airy, Pavanelli, Wander Rogério, Marquez, Audrey de Souza, Kaminami, Mari Sumigawa, Carlos, Nilson de Jesus, Sano, Ayako, Ono, Mario Augusto, Itano, Eiko Nakagawa
Format: Article
Language:eng ; por
Subjects:
Antibodies, Fungal - immunology
Antibodies, Monoclonal - immunology
Antigens, Fungal - immunology
Chronic Disease
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Humans
Immunoglobulin G - immunology
Molecular Weight
Paracoccidioides - immunology
Paracoccidioides - isolation & purification
Paracoccidioidomycosis - parasitology
TROPICAL MEDICINE
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Summary:Different serum levels of the IgG/IgE for Paracoccidioides brasiliensis high mass molecular (hMM) fraction (~366 kDa) in the acute and chronic forms of the disease have been reported. Considering the nonexistence of hMM fraction investigation involving clinical isolates of P. brasiliensis, the present study aimed to investigate the presence of the hMM fraction (~366 kDa) in cell free antigens (CFA) from P. brasiliensis clinical isolates. CFA from 10 clinical isolates and a reference strain (Pb18) were submitted to SDS-polyacrylamide gel electrophoresis (SDS-PAGE) followed by gel image capturing and densitometer analysis. Additionally, CFA from 20 isolates and Pb18 were analyzed by capture ELISA (cELISA) using polyclonal (polAb) or monoclonal (mAb) antibodies to the hMM fraction. The presence of the hMM component was observed in CFA of all samples analyzed by SDS-PAGE/densitometry and by cELISA. In addition, Pearson's correlation test demonstrated stronger coefficients between hMM fraction levels using pAb and mAb (R = 0.853) in cELISA. The soluble hMM fraction was present in all the P. brasiliensis clinical isolates analyzed and the reference strain Pb18, which could be used as a source of this antigen. The work also introduces for first time, the cELISA method for P. brasiliensis hMM fraction detection. Analysis also suggests that detection is viable using polAb or mAb and this methodology may be useful for future investigation of the soluble hMM fraction (~366 kDa) in sera from PCM patients.
ISSN:0037-8682
1678-9849
1678-9849
0037-8682
DOI:10.1590/S0037-86822010000500011