EVALUATION OF THE HUMAN IMMUNODEFICIENCY VIRUS TYPE 1 AND 2 ANTIBODIES DETECTION IN DRIED WHOLE BLOOD SPOTS (DBS) SAMPLES

Human Immunodeficiency Virus Type 1 and 2 antibodies detection was performed in 457 dried whole blood spots samples (S&S 903). Q-Preven HIV 1+2 was the screening test used. The results were compared with the gold standard serum tests by ELISA (Cobas Core e Axsym HIV1/2 gO) and immunofluorescence...

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Bibliographic Details
Published in:Revista do Instituto de Medicina Tropical de São Paulo 2008-05, Vol.50 (3), p.151-156
Main Authors: CAUDURO DE CASTRO, Andréa, DOS ANJOS BORGES, Luiz Gustavo, DA SILVA DE SOUZA, Ricardo, GRUDZINSKI, Melina, D'AZEVEDO, Pedro Alves
Format: Article
Language:eng ; por
Subjects:
Adolescent
Adult
Aged
Biological and medical sciences
Blood Specimen Collection - instrumentation
Blood Specimen Collection - methods
Child
Child, Preschool
Enzyme-Linked Immunosorbent Assay
Filtration
Fluorescent Antibody Technique, Indirect
General aspects
HIV Antibodies - blood
HIV Infections - diagnosis
HIV Infections - virology
HIV-1 - immunology
HIV-2 - immunology
Human viral diseases
Humans
Immunodeficiencies
Immunodeficiencies. Immunoglobulinopathies
Immunopathology
Infant
Infectious diseases
Medical sciences
Middle Aged
Sensitivity and Specificity
TROPICAL MEDICINE
Viral diseases
Viral diseases of the lymphoid tissue and the blood. Aids
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Summary:Human Immunodeficiency Virus Type 1 and 2 antibodies detection was performed in 457 dried whole blood spots samples (S&S 903). Q-Preven HIV 1+2 was the screening test used. The results were compared with the gold standard serum tests by ELISA (Cobas Core e Axsym HIV1/2 gO) and immunofluorescence was the definitive confirmatory test. The samples were obtained from the Hospital Nossa Senhora da Conceição in Porto Alegre, RS - Brazil, through whole blood transfer to filter paper card and sent to Caxias do Sul, RS-Brazil where the tests were performed. The dried whole blood spot stability was evaluated with two different panels. The first one was composed of five negative and five positive samples stored at room temperature, 4 degrees C, -20 degrees C and -70 degrees C, while the second was composed of two negative and three positive samples stored at 37 degrees C (humidity
ISSN:0036-4665
1678-9946
1678-9946
0036-4665
DOI:10.1590/S0036-46652008000300004