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An immunofluorescence test for diagnosis of ophthalmic herpes in a mouse corneal model
Herpes simplex virus type 1 (HSV-1) ophthalmic disease is the most common cause of corneal blindness in humans world-wide. Current culture techniques for HSV take several days and commercially available HSV laboratory based diagnostic techniques vary in sensitivity. Our study was conducted to evalua...
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Published in: | Revista do Instituto de Medicina Tropical de São Paulo 2007-03, Vol.49 (2), p.87-92 |
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creator | PEREIRA, Silvia Regina Ferreira Goncalves CAMARA, Fernando Portela GUIMARAES, Maria Angélica Arpon Marandino VIEIRA NETO, Leonardo SEGENREICH, Daniel GUIMARAES, Antonio Carlos Da Costa CHAGAS, Vera Lucia Antunes |
description | Herpes simplex virus type 1 (HSV-1) ophthalmic disease is the most common cause of corneal blindness in humans world-wide. Current culture techniques for HSV take several days and commercially available HSV laboratory based diagnostic techniques vary in sensitivity. Our study was conducted to evaluate the use of a quicker and simpler method to herpes ophthalmic diagnosis. Corneal smears were made by firm imprints of infected mouse eyes to glass slides, after smears were fixated with cold acetone, and an indirect immunofluorescence (IIF) method was performed using monoclonal antibodies in a murine model of ophthalmic herpes. Eye swabs from infected mice were inoculated in Vero cells for virus isolation. Cytology and histology of the eye were also performed, using hematoxylin-eosin routine. Mouse eyes were examined by slit-lamp biomicroscopy for evidence of herpetic disease at various times postinoculation. We made a comparative evaluation of sensitivity, specificity and speed of methods for laboratory detection of HSV. Our results indicate that this IIF method is quick, sensitive, specific and can be useful in the diagnosis of ophthalmic herpes as demonstrated in an animal model. |
doi_str_mv | 10.1590/S0036-46652007000200004 |
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Current culture techniques for HSV take several days and commercially available HSV laboratory based diagnostic techniques vary in sensitivity. Our study was conducted to evaluate the use of a quicker and simpler method to herpes ophthalmic diagnosis. Corneal smears were made by firm imprints of infected mouse eyes to glass slides, after smears were fixated with cold acetone, and an indirect immunofluorescence (IIF) method was performed using monoclonal antibodies in a murine model of ophthalmic herpes. Eye swabs from infected mice were inoculated in Vero cells for virus isolation. Cytology and histology of the eye were also performed, using hematoxylin-eosin routine. Mouse eyes were examined by slit-lamp biomicroscopy for evidence of herpetic disease at various times postinoculation. We made a comparative evaluation of sensitivity, specificity and speed of methods for laboratory detection of HSV. Our results indicate that this IIF method is quick, sensitive, specific and can be useful in the diagnosis of ophthalmic herpes as demonstrated in an animal model.</description><identifier>ISSN: 0036-4665</identifier><identifier>ISSN: 1678-9946</identifier><identifier>EISSN: 1678-9946</identifier><identifier>EISSN: 0036-4665</identifier><identifier>DOI: 10.1590/S0036-46652007000200004</identifier><identifier>PMID: 17505665</identifier><identifier>CODEN: RMTSAE</identifier><language>eng ; por</language><publisher>São Paulo: Instituto de Medicina Tropical de São Paulo</publisher><subject>Animals ; Antigens, Viral - analysis ; Biological and medical sciences ; Cercopithecus aethiops ; Disease Models, Animal ; Fluorescent Antibody Technique, Indirect ; General aspects ; Herpesvirus 1, Human - immunology ; Human viral diseases ; Humans ; Infectious diseases ; Keratitis, Herpetic - diagnosis ; Male ; Medical sciences ; Mice ; Mice, Inbred BALB C ; Reproducibility of Results ; Time Factors ; TROPICAL MEDICINE ; Vero Cells ; Viral diseases ; Viral diseases with cutaneous or mucosal lesions and viral diseases of the eye</subject><ispartof>Revista do Instituto de Medicina Tropical de São Paulo, 2007-03, Vol.49 (2), p.87-92</ispartof><rights>2007 INIST-CNRS</rights><rights>Copyright Instituto de Medicina Tropical de Sao Paulo Mar/Apr 2007</rights><rights>This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3714-232236e87ebc92567e96badaea530c870e59c72586954f60864658f0c662b9ab3</citedby><cites>FETCH-LOGICAL-c3714-232236e87ebc92567e96badaea530c870e59c72586954f60864658f0c662b9ab3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,315,786,790,891,24178,27957,27958</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18723156$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17505665$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>PEREIRA, Silvia Regina Ferreira Goncalves</creatorcontrib><creatorcontrib>CAMARA, Fernando Portela</creatorcontrib><creatorcontrib>GUIMARAES, Maria Angélica Arpon Marandino</creatorcontrib><creatorcontrib>VIEIRA NETO, Leonardo</creatorcontrib><creatorcontrib>SEGENREICH, Daniel</creatorcontrib><creatorcontrib>GUIMARAES, Antonio Carlos Da Costa</creatorcontrib><creatorcontrib>CHAGAS, Vera Lucia Antunes</creatorcontrib><title>An immunofluorescence test for diagnosis of ophthalmic herpes in a mouse corneal model</title><title>Revista do Instituto de Medicina Tropical de São Paulo</title><addtitle>Rev Inst Med Trop Sao Paulo</addtitle><description>Herpes simplex virus type 1 (HSV-1) ophthalmic disease is the most common cause of corneal blindness in humans world-wide. Current culture techniques for HSV take several days and commercially available HSV laboratory based diagnostic techniques vary in sensitivity. Our study was conducted to evaluate the use of a quicker and simpler method to herpes ophthalmic diagnosis. Corneal smears were made by firm imprints of infected mouse eyes to glass slides, after smears were fixated with cold acetone, and an indirect immunofluorescence (IIF) method was performed using monoclonal antibodies in a murine model of ophthalmic herpes. Eye swabs from infected mice were inoculated in Vero cells for virus isolation. Cytology and histology of the eye were also performed, using hematoxylin-eosin routine. Mouse eyes were examined by slit-lamp biomicroscopy for evidence of herpetic disease at various times postinoculation. We made a comparative evaluation of sensitivity, specificity and speed of methods for laboratory detection of HSV. 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Current culture techniques for HSV take several days and commercially available HSV laboratory based diagnostic techniques vary in sensitivity. Our study was conducted to evaluate the use of a quicker and simpler method to herpes ophthalmic diagnosis. Corneal smears were made by firm imprints of infected mouse eyes to glass slides, after smears were fixated with cold acetone, and an indirect immunofluorescence (IIF) method was performed using monoclonal antibodies in a murine model of ophthalmic herpes. Eye swabs from infected mice were inoculated in Vero cells for virus isolation. Cytology and histology of the eye were also performed, using hematoxylin-eosin routine. Mouse eyes were examined by slit-lamp biomicroscopy for evidence of herpetic disease at various times postinoculation. We made a comparative evaluation of sensitivity, specificity and speed of methods for laboratory detection of HSV. Our results indicate that this IIF method is quick, sensitive, specific and can be useful in the diagnosis of ophthalmic herpes as demonstrated in an animal model.</abstract><cop>São Paulo</cop><pub>Instituto de Medicina Tropical de São Paulo</pub><pmid>17505665</pmid><doi>10.1590/S0036-46652007000200004</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Animals Antigens, Viral - analysis Biological and medical sciences Cercopithecus aethiops Disease Models, Animal Fluorescent Antibody Technique, Indirect General aspects Herpesvirus 1, Human - immunology Human viral diseases Humans Infectious diseases Keratitis, Herpetic - diagnosis Male Medical sciences Mice Mice, Inbred BALB C Reproducibility of Results Time Factors TROPICAL MEDICINE Vero Cells Viral diseases Viral diseases with cutaneous or mucosal lesions and viral diseases of the eye |
title | An immunofluorescence test for diagnosis of ophthalmic herpes in a mouse corneal model |
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