An immunofluorescence test for diagnosis of ophthalmic herpes in a mouse corneal model

Herpes simplex virus type 1 (HSV-1) ophthalmic disease is the most common cause of corneal blindness in humans world-wide. Current culture techniques for HSV take several days and commercially available HSV laboratory based diagnostic techniques vary in sensitivity. Our study was conducted to evalua...

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Published in:Revista do Instituto de Medicina Tropical de São Paulo 2007-03, Vol.49 (2), p.87-92
Main Authors: PEREIRA, Silvia Regina Ferreira Goncalves, CAMARA, Fernando Portela, GUIMARAES, Maria Angélica Arpon Marandino, VIEIRA NETO, Leonardo, SEGENREICH, Daniel, GUIMARAES, Antonio Carlos Da Costa, CHAGAS, Vera Lucia Antunes
Format: Article
Language:eng ; por
Subjects:
Animals
Antigens, Viral - analysis
Biological and medical sciences
Cercopithecus aethiops
Disease Models, Animal
Fluorescent Antibody Technique, Indirect
General aspects
Herpesvirus 1, Human - immunology
Human viral diseases
Humans
Infectious diseases
Keratitis, Herpetic - diagnosis
Male
Medical sciences
Mice
Mice, Inbred BALB C
Reproducibility of Results
Time Factors
TROPICAL MEDICINE
Vero Cells
Viral diseases
Viral diseases with cutaneous or mucosal lesions and viral diseases of the eye
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Summary:Herpes simplex virus type 1 (HSV-1) ophthalmic disease is the most common cause of corneal blindness in humans world-wide. Current culture techniques for HSV take several days and commercially available HSV laboratory based diagnostic techniques vary in sensitivity. Our study was conducted to evaluate the use of a quicker and simpler method to herpes ophthalmic diagnosis. Corneal smears were made by firm imprints of infected mouse eyes to glass slides, after smears were fixated with cold acetone, and an indirect immunofluorescence (IIF) method was performed using monoclonal antibodies in a murine model of ophthalmic herpes. Eye swabs from infected mice were inoculated in Vero cells for virus isolation. Cytology and histology of the eye were also performed, using hematoxylin-eosin routine. Mouse eyes were examined by slit-lamp biomicroscopy for evidence of herpetic disease at various times postinoculation. We made a comparative evaluation of sensitivity, specificity and speed of methods for laboratory detection of HSV. Our results indicate that this IIF method is quick, sensitive, specific and can be useful in the diagnosis of ophthalmic herpes as demonstrated in an animal model.
ISSN:0036-4665
1678-9946
1678-9946
0036-4665
DOI:10.1590/S0036-46652007000200004