A low-cost, paper-based hybrid capture assay to detect high-risk HPV DNA for cervical cancer screening in low-resource settings

Cervical cancer is a leading cause of cancer death for women in low-resource settings. The World Health Organization recommends that cervical cancer screening programs incorporate HPV DNA testing, but available tests are expensive, require laboratory infrastructure, and cannot be performed at the po...

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Bibliographic Details
Published in:Lab on a chip 2023-01, Vol.23 (3), p.451-465
Main Authors: Smith, Chelsey A, Chang, Megan M, Kundrod, Kathryn A, Novak, Emilie N, Parra, Sonia G, López, Leticia, Mavume, Celda, Lorenzoni, Cesaltina, Maza, Mauricio, Salcedo, Mila P, Carns, Jennifer L, Baker, Ellen, Montealegre, Jane, Scheurer, Michael, Castle, Philip E, Schmeler, Kathleen M, Richards-Kortum, Rebecca R
Format: Article
Language:English
Subjects:
Assaying
Cancer
Cervical cancer
Chemistry
DNA
DNA testing
Early Detection of Cancer - methods
Equipment costs
Female
Human papillomavirus
Humans
Laboratories
Low cost
Medical screening
Optimization
Papillomaviridae - genetics
Papillomavirus Infections - diagnosis
Performance evaluation
Reagents
User-Computer Interface
Uterine Cervical Neoplasms - diagnosis
Uterine Cervical Neoplasms - prevention & control
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Summary:Cervical cancer is a leading cause of cancer death for women in low-resource settings. The World Health Organization recommends that cervical cancer screening programs incorporate HPV DNA testing, but available tests are expensive, require laboratory infrastructure, and cannot be performed at the point-of-care. We developed a two-dimensional paper network (2DPN), hybrid-capture, signal amplification assay and a point-of-care sample preparation protocol to detect high-risk HPV DNA from exfoliated cervical cells within an hour. The test does not require expensive equipment and has an estimated cost of
ISSN:1473-0197
1473-0189
DOI:10.1039/d2lc00885h