One-pot biocatalytic synthesis of nylon monomers from cyclohexanol using -based concurrent cascade consortia

We developed a multienzyme biocatalytic cascade with high atom efficiency and a self-sufficient redox network for the synthesis of nylon monomers without adding auxiliary enzymes to recycle cofactors. Herein, a cell-based modularization strategy was applied to produce 6-aminohexanoic acid and -capro...

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Published in:Green chemistry : an international journal and green chemistry resource : GC 2021-11, Vol.23 (23), p.9447-9453
Main Authors: Sarak, Sharad, Khobragade, Taresh P, Jeon, Hyunwoo, Pagar, Amol D, Giri, Pritam, Lee, Somin, Yun, Hyungdon
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Summary:We developed a multienzyme biocatalytic cascade with high atom efficiency and a self-sufficient redox network for the synthesis of nylon monomers without adding auxiliary enzymes to recycle cofactors. Herein, a cell-based modularization strategy was applied to produce 6-aminohexanoic acid and -caprolactam from cyclohexanol. The designed consortia of E. coli containing the respective modules was found to produce >97% of 6-aminohexanoic acid (from 100 mM cyclohexanol) and 28% of caprolactam (from 10 mM cyclohexanol) with 2-fold excess of propylamine in a one-pot reaction. The use of cheaper substrates and co-substrates makes it an economically beneficial approach for the production of nylon precursors. We developed a multienzyme biocatalytic cascade with high atom efficiency and a self-sufficient redox network for the synthesis of nylon monomers without adding auxiliary enzymes to recycle cofactors.
ISSN:1463-9262
1463-9270
DOI:10.1039/d1gc03056f