Direct and selective metal-free N-arylation of adenosine residues for simple fluorescence labeling of DNA and RNA

We have developed an unprecedented transition metal-free approach for the direct fluorescence turn-on labeling of natural oligonucleotides through selective N 6 -arylation of adenosine moieties. This method allows the simple and direct fluorescence labeling of natural unmodified DNA and RNA, but is...

Full description

Saved in:
Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2021-06, Vol.57 (44), p.545-5453
Main Authors: Ravi Kumara, Guralamatta Siddappa, Pandith, Anup, Seo, Young Jun
Format: Article
Language:
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We have developed an unprecedented transition metal-free approach for the direct fluorescence turn-on labeling of natural oligonucleotides through selective N 6 -arylation of adenosine moieties. This method allows the simple and direct fluorescence labeling of natural unmodified DNA and RNA, but is dependent on the secondary structure, favoring single-stranded structures. We have developed an unprecedented transition metal-free approach for the direct fluorescence turn-on labeling of natural oligonucleotides through selective N 6 -arylation of adenosine moieties.
ISSN:1359-7345
1364-548X
DOI:10.1039/d1cc02069b