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Nitric oxide-dependent biodegradation of graphene oxide reduces inflammation in the gastrointestinal tract
Understanding the biological fate of graphene-based materials such as graphene oxide (GO) is crucial to assess adverse effects following intentional or inadvertent exposure. Here we provide first evidence of biodegradation of GO in the gastrointestinal tract using zebrafish as a model. Raman mapping...
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Published in: | Nanoscale 2020-08, Vol.12 (32), p.1673-16737 |
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Main Authors: | , , , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Understanding the biological fate of graphene-based materials such as graphene oxide (GO) is crucial to assess adverse effects following intentional or inadvertent exposure. Here we provide first evidence of biodegradation of GO in the gastrointestinal tract using zebrafish as a model. Raman mapping was deployed to assess biodegradation. The degradation was blocked upon knockdown of
nos2a
encoding the inducible nitric oxide synthase (iNOS) or by pharmacological inhibition of NOS using
l
-NAME, demonstrating that the process was nitric oxide (NO)-dependent. NO-dependent degradation of GO was further confirmed
in vitro
by combining a superoxide-generating system, xanthine/xanthine oxidase (X/XO), with an NO donor (PAPA NONOate), or by simultaneously producing superoxide and NO by decomposition of SIN-1. Finally, by using the transgenic strain
Tg
(
mpx
:eGFP) to visualize the movement of neutrophils, we could show that inhibition of the degradation of GO resulted in increased neutrophil infiltration into the gastrointestinal tract, indicative of inflammation.
Graphene oxide (GO) undergoes nitric oxide (NO)-dependent degradation leading to reduced infiltration of polymorphonuclear cells (PMNs) in the GI tract. |
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ISSN: | 2040-3364 2040-3372 2040-3372 |
DOI: | 10.1039/d0nr03675g |